miR-Intess: classification

confident novel loci of miRBase miRNAs

miRBaseNonMapped_cloningOK_randfoldOK (1 loci)

rno-mir-31-as_novel-as

Cloning frequencies
absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
rno-mir-31-as_novel-as 3arm	7	0	3	8	2	2	2	8	1	3
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
rno-mir-31-as_novel-as 3arm	0.000	0	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000

block1861583 (Antisense miRBase rno-mir-31-as) [miRBaseNonMapped_cloningOK_randfoldOK]

Hairpin parameters
miRNA	randfold	RNA	rep	GC min/max	len min/max/mir_range	5'var	3'var	reads	reads edited	libs	libs edited	base distance	loop distance	position	locations	exon distance	unpaired	max bulge	total reads	total libs	dicer	drosha
miRBaseAS rno-mir-31-as	0.001	no	no	0.50/0.56	18/22/0.81	0.0	0.0	21	0	9	0	32	5	3arm	1	nd	0.15	2	36	9	na	na

		reads	miRBase family seed
seed	-------------------------------------------------------------------UAUGCCA--------------------------------------------	27	novel
seed	-----------------------------------------------------------------GCUAUGC----------------------------------------------	5	novel
seed	---------------------------------------------------------------------UGCCAGC------------------------------------------	3	novel
seed	------------------------------------------------------------------CUAUGCC---------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	------------------------------------------------------------------CTATGCCAGCATCTTGCCTC--------------------------------	20	7	1	4	1	1	1	7	-	3
	--------------------------------------------------------------------ATGCCAGCATCTTGCCTC--------------------------------	18	-	1	1	-	-	-	1	-	-
	----------------------------------------------------------------AGCTATGCCAGCATCTTGCCTC--------------------------------	22	-	-	1	1	-	1	-	-	-
	----------------------------------------------------------------AGCTATGCCAGCATCTTG------------------------------------	18	-	-	2	-	-	-	-	-	-
	------------------------------------------------------------------CTATGCCAGCATCTTGCC----------------------------------	18	-	1	-	-	-	-	-	1	-
	-----------------------------------------------------------------GCTATGCCAGCATCTTGCCT---------------------------------	20	-	-	-	-	1	-	-	-	-
rat	TGGCCAAGCTGCTGTCAGACAGGAAAGATGGCAATATGTTGGCATAGCAGGTTCTCAGTTCAACAGCTATGCCAGCATCTTGCCTCCTCTCCAGTTCCAAGTTTCAGGAGCAATACCA
human	---------------------GGAAAGATGGCAATATGTTGGCATAGCAGGTTCCCAGTTCAACAGCTATGCCAGCATCTTGCCTCCTCTCC--------------------------
mouse	---------------------GGAAAGATGGCAATATGTTGGCATAGCAGGTTCTCAGTTCAACAGCTATGCCAGCATCTTGCCTCCTCTCC--------------------------
	******************************* ***********************************
	...........<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<...........	ENSRNOT00000053729 ENSRNOG00000035606 rno-mir-31
rat	.((....(((.(((...(((.(((.((..(((((.(((((((((((((..(((........))).))))))))))))).)))))..)).))).)))........))).)))....)).	0.780 -45.60
human	(((.((..(((((.(((((((((((((..(((........))).))))))))))))).)))))..)).)))	0.963 -34.90
mouse	(((.((..(((((.(((((((((((((..(((........))).))))))))))))).)))))..)).)))	0.999 -34.90

rat	chromosome:5:108497643:108497760:1	Opposite_strand\|Exonic_non-coding\|ENSRNOT00000053729\|ENSRNOG00000035606 ## ENSRNOG00000035606\|miRNA\|rno-mir-31\|rno-mir-31 [Source:miRBase;Acc:MI0000872] ## {ASMIR: rno-mir-31-as}
human	chromosome:9:21502114:21502184:1	Opposite_strand\|Intronic_non-coding\|ENST00000304425\|ENSG00000171889\|protein_coding\|Putative uncharacterized protein LOC554202 [Source:UniProtKB/Swiss-Prot;Acc:Q8WTY6] ## Opposite_strand\|Exonic_non-coding\|ENST00000362307\|ENSG00000199177\|miRNA\|hsa-mir-31 [Source:miRBase;Acc:MI0000089] ## {ASMIR: hsa-mir-31-as}
mouse	chromosome:4:88556476:88556546:1	Opposite_strand\|Exonic_non-coding\|ENSMUST00000083474\|ENSMUSG00000065408\|miRNA\|mmu-mir-31 [Source:miRBase;Acc:MI0000579] ## {ASMIR: mmu-mir-31-as}

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confident novel loci of miRBase miRNAs:

Page 1

miR-Intess™

rno-mir-31-as_novel-as

block1861583 (Antisense miRBase rno-mir-31-as) [miRBaseNonMapped_cloningOK_randfoldOK]