miR-Intess™

confident novel miRNAs

MANUAL_level1 (6 loci)

sblock651_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock651_novel 5arm	0.250	1.667	0	0	0	0	0	0	0	0
sblock651_novel 3arm	74.667	85.269	0	0.111	0.200	0	0	0	0	0.111
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock651_novel 5arm	0.000	0.000	0	0	0	0	0	0	0	0
sblock651_novel 3arm	0.002	0.004	0	0.000	0.000	0	0	0	0	0.000

sblock651 [MANUAL_level1]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.40/0.53

18/23/0.65

0.0 0.0

1.0 1.4

6 882

0 0

2 5

0 0

21 18

6 7

5arm 3arm

4 9

nd nd

0.17 0.10

2 1

1087

5

1

2

Families

Member of family novel15 (seed GUAUAAC): sblock647_novel, sblock650_novel, sblock651_novel, sblock652_novel, sblock649_cand

		reads	miRBase family seed
seed	---------------------------------------------------------------GUAUAAC--------------------------------	1066	novel
seed	------------------------------------------------------------------UAACCAA-----------------------------	8	novel
seed	----------------------------------------------------------------UAUAACC-------------------------------	6	novel
seed	----------------------GUCAGGC-------------------------------------------------------------------------	6	novel
seed	-------------------------------------------------------------GGGUAUA----------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T3S1	T5S2
	--------------------------------------------------------------GGTATAACCAAAGCCCGACTGA------------------	22	139	288	-	1	-
	--------------------------------------------------------------GGTATAACCAAAGCCCGAC---------------------	19	193	131	1	-	1
	--------------------------------------------------------------GGTATAACCAAAGCCCGACT--------------------	20	131	42	-	-	-
	--------------------------------------------------------------GGTATAACCAAAGCCCGACTG-------------------	21	59	27	-	-	-
	--------------------------------------------------------------GGTATAACCAAAGCCCGA----------------------	18	17	19	-	-	-
	--------------------------------------------------------------GGTATAACCAAAGCCCGACTGAA-----------------	23	7	10	-	-	-
	-----------------------------------------------------------------ATAACCAAAGCCCGACTGA------------------	19	1	4	-	-	-
	---------------------AGTCAGGCTACTGGTTATA--------------------------------------------------------------	19	-	3	-	-	-
	---------------------------------------------------------------GTATAACCAAAGCCCGAC---------------------	18	2	1	-	-	-
	-----------------------------------------------------------------ATAACCAAAGCCCGACTG-------------------	18	2	1	-	-	-
	---------------------------------------------------------------GTATAACCAAAGCCCGACTGA------------------	21	2	1	-	-	-
	---------------------AGTCAGGCTACTGGTTATAT-------------------------------------------------------------	20	-	1	-	-	-
	---------------------AGTCAGGCTACTGGTTAT---------------------------------------------------------------	18	1	-	-	-	-
	------------------------------------------------------------TGGGTATAACCAAAGCCCGAC---------------------	21	-	1	-	-	-
	---------------------AGTCAGGCTACTGGTTATATTCC----------------------------------------------------------	23	-	1	-	-	-
rat	TCTGCAGGGAAAGATCTGATCAGTCAGGCTACTGGTTATATTCCAGGACTTGTCCAGTCCTGGGTATAACCAAAGCCCGACTGAATGGTGTTAAATAAAAGA
mouse	-------------------TCAGTCAGGCTTCTGGCTATATTCCAGGACATACCTGGTCCTGGGTATAACCAAAGCCCGACTGT------------------
	*********** **** ************* *  *  ***************************
rat	(((.......((.(((...((((((.((((..((((((((.(((((((((.....))))))))))))))))).)))).))))))..))).)).......)))	0.470 -41.94
mouse	.(((((.(((((.(((.((((.((((((((.......)))))))))))).)))))))).))))).	1.000 -33.90

rat	chromosome:1:115352667:115352768:-1	intergenic
mouse	chromosome:7:68884198:68884339:-1	intergenic

sblock652_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock652_novel 5arm	8.286	4.857	0	0	0	0	0	0	0	0
sblock652_novel 3arm	74.667	85.537	0	0.111	0.200	0	0	0	0	0.111
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock652_novel 5arm	0.000	0.000	0	0	0	0	0	0	0	0
sblock652_novel 3arm	0.002	0.004	0	0.000	0.000	0	0	0	0	0.000

sblock652 [MANUAL_level1]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.43/0.53

18/23/0.63

0.0 0.5 0.0

1.3 1.0 1.4

58 2 882

0 0 0

2 1 5

0 0 0

24 32 23

6 -1 7

5arm 3arm 3arm

7 8 9

nd nd nd

0.17 0.05 0.10

2 1 1

1175

5

1

2

Families

Member of family novel15 (seed GUAUAAC): sblock647_novel, sblock650_novel, sblock651_novel, sblock652_novel, sblock649_cand

		reads	miRBase family seed
seed	------------------------------------------------------------------GUAUAAC-------------------------------------	1066	novel
seed	-------------------------GUCAGGC------------------------------------------------------------------------------	79	novel
seed	---------------------------CAGGCUG----------------------------------------------------------------------------	12	novel
seed	---------------------------------------------------------------------UAACCAA----------------------------------	8	novel
seed	-------------------------------------------------------------------UAUAACC------------------------------------	6	novel
seed	-----------------------------GGCUGCU--------------------------------------------------------------------------	1	novel
seed	-----------------------------------------------------------GUCCUGG--------------------------------------------	1	novel
seed	----------------------------------------------------------GGUCCUG---------------------------------------------	1	novel
seed	----------------------------------------------------------------GGGUAUA---------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T3S1	T5S2
	-----------------------------------------------------------------GGTATAACCAAAGCCCGACTGA-----------------------	22	139	288	-	1	-
	-----------------------------------------------------------------GGTATAACCAAAGCCCGAC--------------------------	19	193	131	1	-	1
	-----------------------------------------------------------------GGTATAACCAAAGCCCGACT-------------------------	20	131	42	-	-	-
	-----------------------------------------------------------------GGTATAACCAAAGCCCGACTG------------------------	21	59	27	-	-	-
	-----------------------------------------------------------------GGTATAACCAAAGCCCGA---------------------------	18	17	19	-	-	-
	------------------------AGTCAGGCTGCTGGTTATA-------------------------------------------------------------------	19	17	15	-	-	-
	------------------------AGTCAGGCTGCTGGTTAT--------------------------------------------------------------------	18	17	3	-	-	-
	-----------------------------------------------------------------GGTATAACCAAAGCCCGACTGAA----------------------	23	7	10	-	-	-
	------------------------AGTCAGGCTGCTGGTTATATTCC---------------------------------------------------------------	23	9	5	-	-	-
	------------------------AGTCAGGCTGCTGGTTATAT------------------------------------------------------------------	20	4	2	-	-	-
	------------------------AGTCAGGCTGCTGGTTATATTC----------------------------------------------------------------	22	3	3	-	-	-
	--------------------------------------------------------------------ATAACCAAAGCCCGACTGA-----------------------	19	1	4	-	-	-
	--------------------------TCAGGCTGCTGGTTATATTC----------------------------------------------------------------	20	2	2	-	-	-
	--------------------------TCAGGCTGCTGGTTATATTCC---------------------------------------------------------------	21	2	2	-	-	-
	--------------------------TCAGGCTGCTGGTTATAT------------------------------------------------------------------	18	3	1	-	-	-
	------------------------------------------------------------------GTATAACCAAAGCCCGAC--------------------------	18	2	1	-	-	-
	------------------------------------------------------------------GTATAACCAAAGCCCGACTGA-----------------------	21	2	1	-	-	-
	--------------------------------------------------------------------ATAACCAAAGCCCGACTG------------------------	18	2	1	-	-	-
	---------------------------------------------------------TGGTCCTGGGTATAACCAA----------------------------------	19	-	1	-	-	-
	----------------------------AGGCTGCTGGTTATATTCC---------------------------------------------------------------	19	1	-	-	-	-
	---------------------------------------------------------------TGGGTATAACCAAAGCCCGAC--------------------------	21	-	1	-	-	-
	------------------------AGTCAGGCTGCTGGTTATATT-----------------------------------------------------------------	21	-	1	-	-	-
	----------------------------------------------------------GGTCCTGGGTATAACCAAAG--------------------------------	20	-	1	-	-	-
rat	TTGTCTGCAAGGAGAGATCTGATCAGTCAGGCTGCTGGTTATATTCCAGGACTAATCTGGTCCTGGGTATAACCAAAGCCCGACTGAATGGTGTTAAATAAAAGAACAGT
mouse	----------------------TCAGTCAGGCTTCTGGCTATATTCCAGGACATACCTGGTCCTGGGTATAACCAAAGCCCGACT-----------------------GT
	*********** **** *************  * *****************************                       **
rat	((((((.......((.(((...((((((.((((..((((((((.(((((((((.....))))))))))))))))).)))).))))))..))).)).......))).))).	0.900 -46.04
mouse	.(((((.(((((.(((.((((.((((((((.......)))))))))))).)))))))).))))                       ).	1.000 -33.90

rat	chromosome:1:115354288:115354397:-1	intergenic
mouse	chromosome:7:68884193:68884342:-1	intergenic

novel_cloningHIGH_multiarm_DicerOK_randfoldOK (5 loci)

sblock10007_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock10007_novel 5arm	7	3	0	0	3	0	0	0	1	0
sblock10007_novel 3arm	16	13	1	0	0	3	3	6	0	3
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock10007_novel 5arm	0.000	0.000	0	0	0.000	0	0	0	0.000	0
sblock10007_novel 3arm	0.001	0.001	0.000	0	0	0.000	0.000	0.000	0	0.000

sblock10007 [novel_cloningHIGH_multiarm_DicerOK_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.002

no

no

0.53/0.67

18/22/0.76

0.9 0.0

0.5 0.7

14 30

0 0

4 7

0 0

22 15

0 2

5arm 3arm

1 1

nd nd

0.11 0.09

2 2

59

9

2

2

		reads	miRBase family seed
seed	-------------------------------------------------------UUCAACC-----------------------------	39	novel
seed	------------------------GCCUCCG------------------------------------------------------------	8	novel
seed	-----------------------GGCCUCC-------------------------------------------------------------	5	novel
seed	--------------------------------------------------------UCAACCU----------------------------	4	novel
seed	---------------------------UCCGCAG---------------------------------------------------------	1	novel
seed	-------------------------------------------------------------CUUAAGG-----------------------	1	novel
seed	---------------------------------------------------------CAACCUU---------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	------------------------------------------------------CTTCAACCTTAAGGGGGCCTC----------------	21	6	3	-	-	1	2	3	-	-
	------------------------------------------------------CTTCAACCTTAAGGGGGCCTCA---------------	22	3	5	-	-	-	-	1	-	1
	------------------------------------------------------CTTCAACCTTAAGGGGGCC------------------	19	3	1	-	-	1	1	-	-	2
	-----------------------GGCCTCCGCAGGGTTGAAGCT-----------------------------------------------	21	2	3	-	2	-	-	-	-	-
	------------------------------------------------------CTTCAACCTTAAGGGGGCCT-----------------	20	1	3	-	-	-	-	1	-	-
	----------------------TGGCCTCCGCAGGGTTGAAGCT-----------------------------------------------	22	3	-	-	-	-	-	-	1	-
	-------------------------------------------------------TTCAACCTTAAGGGGGCCTC----------------	20	-	1	-	-	-	-	1	-	-
	-------------------------------------------------------TTCAACCTTAAGGGGGCCT-----------------	19	1	-	-	-	-	-	-	-	-
	----------------------TGGCCTCCGCAGGGTTGA---------------------------------------------------	18	1	-	-	-	-	-	-	-	-
	------------------------------------------------------CTTCAACCTTAAGGGGGC-------------------	18	-	-	1	-	-	-	-	-	-
	--------------------------CTCCGCAGGGTTGAAGCT-----------------------------------------------	18	1	-	-	-	-	-	-	-	-
	------------------------------------------------------------CCTTAAGGGGGCCTCAGGGAG----------	21	-	-	-	-	1	-	-	-	-
	-------------------------------------------------------TTCAACCTTAAGGGGGCC------------------	18	1	-	-	-	-	-	-	-	-
	-----------------------GGCCTCCGCAGGGTTGAA--------------------------------------------------	18	-	-	-	1	-	-	-	-	-
	--------------------------------------------------------TCAACCTTAAGGGGGCCTCAGG-------------	22	1	-	-	-	-	-	-	-	-
rat	CCATCACCACACGACTGGATTTTGGCCTCCGCAGGGTTGAAGCTGCTAGAGAAGCTTCAACCTTAAGGGGGCCTCAGGGAGAGAGGAGTGG
human	-------------------TTTCGGCCTCTGCAGGGTGTAAGCTGCTAAAGAAGCTTCAACCTTAAGGGGGCCTCA---------------
mouse	--------------------TTTGGCCTCCGCGGGGTTGAAGCTGCTAGAGACGCTTCAACCTTAAGGGGGCCTCA---------------
	** ****** ** ****  ********* *** ***********************
	...................................................................<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<	ENSRNOT00000010876 ENSRNOG00000008225
rat	((((..((......((((.....(((((((..((((((((((((........))))))))))))..))))))))))).......)).))))	0.810 -40.02
human	....(((((((..(((((..(((((........)))))..)))))..)))))))...	1.000 -25.60
mouse	...(((((((..(((((((((((..........)))))))))))..)))))))...	1.000 -31.90

rat	chromosome:6:76163538:76163628:-1	Opposite_strand|Boundary_non-coding|ENSRNOT00000010876|ENSRNOG00000008225 ## intergenic ## ENSRNOG00000008225|pseudogene||
human	chromosome:14:35348148:35348278:-1	Same_strand|Boundary_non-coding|ENST00000389698|ENSG00000174373|protein_coding|GTPase-activating Rap/Ran-GAP domain-like 1 (GAP-related-interacting partner to E12)(GRIPE)(Tuberin-like protein 1) [Source:UniProtKB/Swiss-Prot;Acc:Q6GYQ0] ## {SimpF: oe = 0.78 0 CpG,rank = 1 -1 FirstEF}
mouse	chromosome:12:56922030:56922160:-1	Same_strand|Boundary_non-coding|ENSMUST00000110687|ENSMUSG00000021027|protein_coding|GTPase activating RANGAP domain-like 1 Gene [Source:MGI Symbol;Acc:MGI:1931050] ## Opposite_strand|Boundary_non-coding|ENSMUST00000061934|ENSMUSG00000046747|pseudogene| ## {SimpF: oe = 0.97 1 CpG, 1 Eponine}

sblock10242_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock10242_novel 5arm	2	0	19	8	15	17	2	6	7	3
sblock10242_novel 3arm	0	1	3	3	9	3	0	0	1	2
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock10242_novel 5arm	0.000	0	0.001	0.000	0.001	0.001	0.000	0.000	0.000	0.000
sblock10242_novel 3arm	0	0.000	0.000	0.000	0.000	0.000	0	0	0.000	0.000

sblock10242 [novel_cloningHIGH_multiarm_DicerOK_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.44/0.54

18/24/0.75

0.0 0.0

1.6 0.0

64 9

0 0

9 7

0 0

25 26

0 3

5arm 3arm

1 1

nd nd

0.13 0.14

3 2

101

10

3

1

		reads	miRBase family seed
seed	--------------------------CAUAGAA---------------------------------------------------------------------------------	78	novel
seed	--------------------------------------------------------------------CUGUGGA---------------------------------------	17	novel
seed	-------------------------------------------------------------------ACUGUGG----------------------------------------	3	miR-881
seed	---------------------------------------------------------------------UGUGGAG--------------------------------------	2	novel
seed	---------------------------AUAGAAG--------------------------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	-------------------------CCATAGAAGTCATCCCACAGTG-------------------------------------------------------------------	22	2	-	4	2	4	6	-	-	1	-
	-------------------------CCATAGAAGTCATCCCACAGTGCC-----------------------------------------------------------------	24	-	-	4	-	2	6	1	1	1	-
	-------------------------------------------------------------------ACTGTGGAGGGTTTCTATGTC--------------------------	21	-	1	3	2	4	1	-	-	-	1
	-------------------------CCATAGAAGTCATCCCACA----------------------------------------------------------------------	19	-	-	4	-	3	1	1	1	-	-
	-------------------------CCATAGAAGTCATCCCACAG---------------------------------------------------------------------	20	-	-	2	-	2	1	-	1	3	1
	-------------------------CCATAGAAGTCATCCCAC-----------------------------------------------------------------------	18	-	-	3	-	1	1	-	2	2	-
	-------------------------CCATAGAAGTCATCCCACAGT--------------------------------------------------------------------	21	-	-	2	2	3	-	-	-	-	1
	-------------------------CCATAGAAGTCATCCCACAGTGC------------------------------------------------------------------	23	-	-	-	4	-	2	-	1	-	-
	-------------------------------------------------------------------ACTGTGGAGGGTTTCTATG----------------------------	19	-	-	-	-	1	-	-	-	-	1
	--------------------------------------------------------------------CTGTGGAGGGTTTCTATGTC--------------------------	20	-	-	-	-	1	1	-	-	-	-
	------------------------------------------------------------------CACTGTGGAGGGTTTCTATGTC--------------------------	22	-	-	-	-	1	1	-	-	-	-
	-------------------------------------------------------------------ACTGTGGAGGGTTTCTAT-----------------------------	18	-	-	-	1	-	-	-	-	1	-
	-------------------------------------------------------------------ACTGTGGAGGGTTTCTATGTCA-------------------------	22	-	-	-	-	1	-	-	-	-	-
	------------------------------------------------------------------CACTGTGGAGGGTTTCTAT-----------------------------	19	-	-	-	-	1	-	-	-	-	-
	--------------------------CATAGAAGTCATCCCACA----------------------------------------------------------------------	18	-	-	-	-	-	-	-	-	-	1
rat	gcagctggaccttcacccgggctgtCCATAGAAGTCATCCCACAGTGCCttcaaggagggcatcggcACTGTGGAGGGTTTCTATGTCagcctgagtggtacagatccatgctg
	******************************************************************************************************************
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000020178 ENSRNOG00000014879 Ttc7
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000061747 ENSRNOG00000014879 Ttc7
rat	.((((((((.((((((.(((((((..((((((((((...((((((((((...............))))))))))..)))))))))).))))))).))))...)).)))).))))	1.000 -55.66

rat

chromosome:6:10867923:10868036:1

Same_strand|Intronic_coding|ENSRNOT00000061747|ENSRNOG00000014879 ## ENSRNOG00000014879|protein_coding|Ttc7|Ttc7 protein (Fragment). [Source:UniProtKB/TrEMBL;Acc:Q4G027]

sblock12458_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12458_novel 5arm	0	1	0	2	1	0	0	0	2	0
sblock12458_novel 3arm	7	1	0	9	18.500	2	13	26	5	4
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12458_novel 5arm	0	0.000	0	0.000	0.000	0	0	0	0.000	0
sblock12458_novel 3arm	0.000	0.000	0	0.001	0.001	0.000	0.001	0.001	0.000	0.000

sblock12458 [novel_cloningHIGH_multiarm_DicerOK_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.003

no

no

0.50/0.70

18/23/0.73

0.0 0.0

1.3 0.6

6 57

0 0

4 9

0 0

19 19

3 4

5arm 3arm

1 1

nd nd

0.22 0.14

2 2

93

9

1

2

Families

Member of family novel7 (seed AGCCUGC): sblock12458_novel, block710208_cand, block2089837_cand, block2090425_cand, block2188715_cand, block2189069_cand, block2189814_cand, block2190391_cand, block105522_cand

		reads	miRBase family seed
seed	--------------------------------------------------------------AGCCUGC---------------------------------	84	novel
seed	--------------------GCUGUGU---------------------------------------------------------------------------	6	novel
seed	-------------------------------------------------------------CAGCCUG----------------------------------	2	novel
seed	---------------------------------------------------------------GCCUGCU--------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	-------------------------------------------------------------CAGCCTGCTCACACCCAGCCT--------------------	21	2	-	4	8	1	4	10	1	2
	-------------------------------------------------------------CAGCCTGCTCACACCCAGCC---------------------	20	3	1	3	4	-	3	5	2	-
	-------------------------------------------------------------CAGCCTGCTCACACCCAGC----------------------	19	2	-	2	2	-	2	5	2	1
	-------------------------------------------------------------CAGCCTGCTCACACCCAGCCTC-------------------	22	-	-	-	2	1	3	5	-	1
	-------------------AGCTGTGTGTATGCAGGCA----------------------------------------------------------------	19	-	-	2	-	-	-	-	1	-
	-------------------------------------------------------------CAGCCTGCTCACACCCAG-----------------------	18	-	-	-	1	-	-	2	-	-
	------------------------------------------------------------CCAGCCTGCTCACACCCAG-----------------------	19	-	-	-	1	-	1	-	-	-
	-------------------AGCTGTGTGTATGCAGGCAATGG------------------------------------------------------------	23	-	-	-	1	-	-	-	-	-
	--------------------------------------------------------------AGCCTGCTCACACCCAGCCT--------------------	20	-	-	-	1	-	-	-	-	-
	-------------------AGCTGTGTGTATGCAGGCAATG-------------------------------------------------------------	22	-	1	-	-	-	-	-	-	-
	-------------------AGCTGTGTGTATGCAGGC-----------------------------------------------------------------	18	-	-	-	-	-	-	-	1	-
rat	gcatgacagggagtccgggagctgtgtgtatgcaggcaatggagaaggtgttaagtgtttcCAGCCTGCTCACACCCAGCCTCgaacacatccctcattgtt
	******************************************************************************************************
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000042562 ENSRNOG00000031232 Nrp2
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000048645 ENSRNOG00000031232 Nrp2
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000047847 ENSRNOG00000031232 Nrp2
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000043803 ENSRNOG00000031232 Nrp2
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000047568 ENSRNOG00000031232 Nrp2
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000050917 ENSRNOG00000031232 Nrp2
rat	(((....(((((((.((((.((((.((((..((((((..((((((............))))))))))))..)))).)))))))).))...)))))...))).	1.000 -41.50

rat

chromosome:9:61355069:61355170:1

Same_strand|Intronic_coding|ENSRNOT00000047568|ENSRNOG00000031232 ## ENSRNOG00000031232|protein_coding|Nrp2|Neuropilin-2 precursor (Vascular endothelial cell growth factor 165 receptor 2). [Source:UniProtKB/Swiss-Prot;Acc:O35276]

sblock12689_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12689_novel 5arm	568	368	4101	3719	982	488	348	555	1806	1213
sblock12689_novel 3arm	4	2	38	22	6	2	3	6	7	8
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12689_novel 5arm	0.019	0.016	0.222	0.221	0.048	0.037	0.027	0.029	0.119	0.081
sblock12689_novel 3arm	0.000	0.000	0.002	0.001	0.000	0.000	0.000	0.000	0.000	0.001

sblock12689 [novel_cloningHIGH_multiarm_DicerOK_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.35/0.50

18/24/0.83

0.0 0.3

0.0 1.2

7704 45

0 0

10 10

0 0

28 24

5 9

5arm 3arm

1 1

nd nd

0.14 0.00

3 0

14246

10

2

3

		reads	miRBase family seed
seed	-----------------------------GACAGAC-------------------------------------------------------------------------------	13950	novel
seed	------------------------------ACAGACU------------------------------------------------------------------------------	183	novel
seed	---------------------------------------------------------------------UGUAGUA---------------------------------------	62	novel
seed	----------------------------------------------------------------------GUAGUAC--------------------------------------	34	novel
seed	----------------------------UGACAGA--------------------------------------------------------------------------------	12	novel
seed	---------------------------GUGACAG---------------------------------------------------------------------------------	2	miR-542
seed	-------------------------------CAGACUU-----------------------------------------------------------------------------	1	novel
seed	--------------------------------------------------------------------AUGUAGU----------------------------------------	1	novel
seed	-----------------------------------------------------------------------UAGUACU-------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	----------------------------TGACAGACTTAGTACTACATGA-----------------------------------------------------------------	22	399	247	2923	2527	682	342	249	341	1364	890
	----------------------------TGACAGACTTAGTACTACA--------------------------------------------------------------------	19	47	38	360	358	89	54	27	72	130	103
	----------------------------TGACAGACTTAGTACTAC---------------------------------------------------------------------	18	40	26	297	294	85	27	35	53	132	85
	----------------------------TGACAGACTTAGTACTACATG------------------------------------------------------------------	21	30	16	227	210	54	26	22	41	63	58
	----------------------------TGACAGACTTAGTACTACAT-------------------------------------------------------------------	20	36	26	152	174	45	20	11	24	55	41
	----------------------------TGACAGACTTAGTACTACATGATC---------------------------------------------------------------	24	8	9	83	99	13	13	3	18	26	10
	-----------------------------GACAGACTTAGTACTACATGA-----------------------------------------------------------------	21	3	3	36	40	9	4	1	5	28	9
	--------------------------------------------------------------------ATGTAGTACTGAGTCTGTC----------------------------	19	-	1	8	11	-	-	-	-	1	2
	----------------------------TGACAGACTTAGTACTACATGAT----------------------------------------------------------------	23	1	1	6	2	1	-	-	1	3	6
	-----------------------------GACAGACTTAGTACTACATG------------------------------------------------------------------	20	-	-	8	5	1	1	-	-	2	-
	---------------------------------------------------------------------TGTAGTACTGAGTCTGTCGTG-------------------------	21	-	1	6	1	2	1	-	2	2	1
	-----------------------------GACAGACTTAGTACTACA--------------------------------------------------------------------	18	2	-	3	4	-	1	-	-	1	3
	--------------------------------------------------------------------ATGTAGTACTGAGTCTGTCGTG-------------------------	22	1	-	5	2	1	-	-	2	1	1
	---------------------------------------------------------------------TGTAGTACTGAGTCTGTC----------------------------	18	3	-	6	-	1	-	2	-	-	-
	-----------------------------GACAGACTTAGTACTACAT-------------------------------------------------------------------	19	2	-	3	2	-	-	-	-	-	4
	--------------------------------------------------------------------ATGTAGTACTGAGTCTGT-----------------------------	18	-	-	2	5	-	-	-	1	-	2
	--------------------------------------------------------------------ATGTAGTACTGAGTCTGTCGT--------------------------	21	-	-	2	-	2	-	-	-	2	1
	---------------------------GTGACAGACTTAGTACTACAT-------------------------------------------------------------------	21	-	-	1	2	1	-	-	-	1	1
	---------------------------------------------------------------------TGTAGTACTGAGTCTGTCG---------------------------	19	-	-	1	1	-	1	1	-	-	-
	--------------------------------------------------------------------ATGTAGTACTGAGTCTGTCGTGC------------------------	23	-	-	4	-	-	-	-	-	-	-
	---------------------------GTGACAGACTTAGTACTACATG------------------------------------------------------------------	22	-	2	1	-	-	-	-	-	-	-
	--------------------------------------------------------------------ATGTAGTACTGAGTCTGTCG---------------------------	20	-	-	2	-	-	-	-	-	-	1
	--------------------------CGTGACAGACTTAGTACTACAT-------------------------------------------------------------------	22	-	-	-	-	-	-	-	-	-	2
	-----------------------------GACAGACTTAGTACTACATGATC---------------------------------------------------------------	23	-	-	1	-	-	-	-	-	1	-
	--------------------------------------------------------------------ATGTAGTACTGAGTCTGTCGTGCA-----------------------	24	-	-	1	1	-	-	-	-	-	-
	---------------------------GTGACAGACTTAGTACTACA--------------------------------------------------------------------	20	-	-	-	1	-	-	-	-	-	1
	-------------------------------------------------------------------AATGTAGTACTGAGTCTG------------------------------	18	-	-	-	-	-	-	-	1	-	-
	------------------------------ACAGACTTAGTACTACATG------------------------------------------------------------------	19	-	-	-	-	1	-	-	-	-	-
	---------------------------------------------------------------------TGTAGTACTGAGTCTGTCGT--------------------------	20	-	-	1	-	-	-	-	-	-	-
	----------------------------------------------------------------------GTAGTACTGAGTCTGTCGTG-------------------------	20	-	-	-	1	-	-	-	-	-	-
	---------------------------GTGACAGACTTAGTACTACATGA-----------------------------------------------------------------	23	-	-	-	-	1	-	-	-	-	-
	-----------------------------GACAGACTTAGTACTACATGAT----------------------------------------------------------------	22	-	-	-	1	-	-	-	-	-	-
	---------------------------------------------------------------------TGTAGTACTGAGTCTGTCGTGC------------------------	22	-	-	-	-	-	-	-	-	1	-
rat	ctcagaagaaagcctttttctctctgcgTGACAGACTTAGTACTACATGAtccatgttaatgaaactaatgtagtactgagtctgtcgtgcagtgagtaaaaaaatccaaagaga
	*******************************************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000003714 ENSRNOG00000002751 Zdhhc15
rat	(((....((.....((((((((.(((((((((((((((((((((((((....(((....)))......))))))))))))))))))))))))).))).)))))..))....))).	0.430 -47.00

rat

chromosome:X:92588220:92588334:-1

Same_strand|Intronic_coding|ENSRNOT00000003714|ENSRNOG00000002751 ## ENSRNOG00000002751|protein_coding|Zdhhc15|Palmitoyltransferase ZDHHC15 (EC 2.3.1.-) (Zinc finger DHHC domain- containing protein 15) (DHHC-15). [Source:UniProtKB/Swiss-Prot;Acc:Q2TGJ4]

sblock12740_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12740_novel 5arm	0	0	1	1	2	4	10	14	38	72
sblock12740_novel 3arm	0	1	2	0	1	0	5	8	26	21
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12740_novel 5arm	0	0	0.000	0.000	0.000	0.000	0.001	0.001	0.003	0.005
sblock12740_novel 3arm	0	0.000	0.000	0	0.000	0	0.000	0.000	0.002	0.001

sblock12740 [novel_cloningHIGH_multiarm_DicerOK_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.36/0.57

18/23/0.82

0.0 0.0

0.2 1.1

71 51

0 0

8 7

0 0

31 31

1 2

5arm 3arm

1 1

nd nd

0.14 0.10

1 1

206

9

1

1

		reads	miRBase family seed
seed	--------------------------------UCACAAG-------------------------------------------------------------------------------	141	novel
seed	-------------------------------------------------------------------GAACGGC--------------------------------------------	64	novel
seed	---------------------------------CACAAGA------------------------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	-------------------------------TTCACAAGAAGGTGTCTTTCA------------------------------------------------------------------	21	-	1	-	1	2	3	7	19	39
	-------------------------------TTCACAAGAAGGTGTCTTTCATG----------------------------------------------------------------	23	-	-	-	1	-	4	4	8	9
	------------------------------------------------------------------TGAACGGCCCTTGTTGTGAG--------------------------------	20	-	2	-	-	-	2	1	8	8
	------------------------------------------------------------------TGAACGGCCCTTGTTGTGAGG-------------------------------	21	-	-	-	1	-	2	1	8	9
	-------------------------------TTCACAAGAAGGTGTCTTTCAT-----------------------------------------------------------------	22	-	-	1	-	1	1	1	2	13
	------------------------------------------------------------------TGAACGGCCCTTGTTGTGA---------------------------------	19	1	-	-	-	-	-	4	7	4
	-------------------------------TTCACAAGAAGGTGTCTT---------------------------------------------------------------------	18	-	-	-	-	-	-	1	7	5
	-------------------------------TTCACAAGAAGGTGTCTTTC-------------------------------------------------------------------	20	-	-	-	-	-	1	-	2	5
	------------------------------------------------------------------TGAACGGCCCTTGTTGTG----------------------------------	18	-	-	-	-	-	1	2	3	-
	-------------------------------TTCACAAGAAGGTGTCTTT--------------------------------------------------------------------	19	-	-	-	-	1	1	1	-	-
	--------------------------------TCACAAGAAGGTGTCTTTCA------------------------------------------------------------------	20	-	-	-	-	-	-	-	-	1
rat	gccacatggagcaaatctgtgtgcagtgcttTTCACAAGAAGGTGTCTTTCATggggattaaaacatgaacggcccttgttgtgaggagtaatgtacaacatgtaatgcctgtgtggt
	**********************************************************************************************************************
rat	(((((((((.(((.((.((((((((.((((((((((((.((((.(((.((((((..........)))))).))))))).)))))))))))).)))))).)).))..))))))))))))	1.000 -55.70

rat

chromosome:X:154164067:154164184:-1

intergenic

novel_cloningHIGH_mirtron_randfoldOK (3 loci)

block7023_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block7023_novel 3arm	758	660	9	2	26	13	1	7	7	4
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block7023_novel 3arm	0.025	0.029	0.000	0.000	0.001	0.001	0.000	0.000	0.000	0.000

block7023 [novel_cloningHIGH_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.004

no

no

0.57/0.67

18/23/0.78

0.0

0.0

848

0

10

0

10

16

3arm

1

0

0.19

2

1487

10

na

na

		reads	miRBase family seed
seed	--------------------------------------------------------------------------------GAGCACC-----------------------	1460	novel
seed	---------------------------------------------------------------------------------AGCACCA----------------------	10	miR-29a/29b/29c
seed	-------------------------------------------------------------------------------UGAGCAC------------------------	9	novel
seed	----------------------------------------------------------------------------------GCACCAC---------------------	6	miR-770
seed	------------------------------------------------------------------------------CUGAGCA-------------------------	1	novel
seed	-----------------------------------------------------------------------------------CACCACC--------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	-------------------------------------------------------------------------------TGAGCACCACCCCTCTCTCAG----------	21	460	388	4	1	16	5	1	3	5	2
	-------------------------------------------------------------------------------TGAGCACCACCCCTCTCTC------------	19	97	74	2	-	3	4	-	1	-	1
	-------------------------------------------------------------------------------TGAGCACCACCCCTCTCTCA-----------	20	81	84	-	-	4	2	-	1	1	-
	-------------------------------------------------------------------------------TGAGCACCACCCCTCTCT-------------	18	67	66	1	-	1	-	-	2	1	1
	-------------------------------------------------------------------------------TGAGCACCACCCCTCTCTCAGA---------	22	22	27	2	-	2	2	-	-	-	-
	-------------------------------------------------------------------------------TGAGCACCACCCCTCTCTCAGAT--------	23	13	13	-	-	-	-	-	-	-	-
	--------------------------------------------------------------------------------GAGCACCACCCCTCTCTCAG----------	20	6	2	-	-	-	-	-	-	-	-
	------------------------------------------------------------------------------CTGAGCACCACCCCTCTCTCA-----------	21	2	-	-	1	-	-	-	-	-	-
	---------------------------------------------------------------------------------AGCACCACCCCTCTCTCAG----------	19	1	2	-	-	-	-	-	-	-	-
	------------------------------------------------------------------------------CTGAGCACCACCCCTCTC--------------	18	2	-	-	-	-	-	-	-	-	-
	------------------------------------------------------------------------------CTGAGCACCACCCCTCTCTC------------	20	1	1	-	-	-	-	-	-	-	-
	--------------------------------------------------------------------------------GAGCACCACCCCTCTCTC------------	18	-	1	-	-	-	-	-	-	-	-
	---------------------------------------------------------------------------------AGCACCACCCCTCTCTCA-----------	18	1	-	-	-	-	-	-	-	-	-
	---------------------------------------------------------------------------------AGCACCACCCCTCTCTCAGAT--------	21	1	-	-	-	-	-	-	-	-	-
	--------------------------------------------------------------------------------GAGCACCACCCCTCTCTCAGAT--------	22	-	1	-	-	-	-	-	-	-	-
	----------------------------------------------------------------------------------GCACCACCCCTCTCTCAG----------	18	1	-	-	-	-	-	-	-	-	-
	-----------------------------------------------------------------------------TCTGAGCACCACCCCTCTCTCA-----------	22	1	-	-	-	-	-	-	-	-	-
	---------------------------------------------------------------------------------AGCACCACCCCTCTCTCAGA---------	20	-	1	-	-	-	-	-	-	-	-
	------------------------------------------------------------------------------CTGAGCACCACCCCTCTCTCAGA---------	23	1	-	-	-	-	-	-	-	-	-
	------------------------------------------------------------------------------CTGAGCACCACCCCTCTCT-------------	19	1	-	-	-	-	-	-	-	-	-
rat	CTGACTTCCAAGGTGGGAAGAGGGGTGGGGCCCGGGACTGTACCCATGTGAGGACTATTCTTGAGTCCCGCTCTTGTTCTGAGCACCACCCCTCTCTCAGATGGTGGCAG
mouse	------------GTGGGAAGAGGGGTGGGGCCCGGGACTGTACCCATGTGAGGACTATTCTTGAGTCCTGCTCTTGTTCTGAGCACCACCCCTCTCTCAG----------
	******************************************************** *******************************
	>>>>>>>>>>>>>>>>>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>	ENSRNOT00000024072 ENSRNOG00000017893 LOC685297
rat	(((.((.(((...((((.((((((((((.((.((((((.........(((.(((((.......))))))))....)))))).)).))))))))))))))..))).)))))	0.910 -50.32
mouse	.((((.((((((((((.((.((((((.........((.((((((.......))))))))....)))))).)).)))))))))))))).	0.534 -42.72

rat	chromosome:10:14493066:14493175:-1	Same_strand|Boundary_coding|ENSRNOT00000024072|ENSRNOG00000017893 ## ENSRNOG00000017893|protein_coding|LOC685297|
mouse	chromosome:17:25382466:25382615:-1	Same_strand|Boundary_coding|ENSMUST00000056357|ENSMUSG00000047507|protein_coding|BAI1-associated protein 3 Gene [Source:MGI Symbol;Acc:MGI:2685783]

block1311102_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1311102_novel 3arm	37	26	15	11	13	9.500	4	17	23	11
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1311102_novel 3arm	0.001	0.001	0.001	0.001	0.001	0.001	0.000	0.001	0.002	0.001

block1311102 [novel_cloningHIGH_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.002

no

no

0.64/0.72

18/23/0.76

0.0

0.3

67

0

10

0

18

7

3arm

1

0

0.16

3

168

10

na

na

Families

Member of family novel38 (seed CCCUCCC): block1311102_novel, block82260_cand, block2287553_cand

		reads	miRBase family seed
seed	-----------------------------------------------------------------CCCUCCC--------------------------------	159	novel
seed	----------------------------------------------------------------UCCCUCC---------------------------------	6	novel
seed	------------------------------------------------------------------CCUCCCC-------------------------------	3	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	----------------------------------------------------------------TCCCTCCCCCTCACTGCACAG-------------------	21	17	14	8	5	7	1	3	4	7	3
	----------------------------------------------------------------TCCCTCCCCCTCACTGCACAGA------------------	22	5	1	4	-	1	4	-	3	8	3
	----------------------------------------------------------------TCCCTCCCCCTCACTGCAC---------------------	19	6	2	1	2	3	1	-	3	5	1
	----------------------------------------------------------------TCCCTCCCCCTCACTGCACA--------------------	20	4	4	-	3	2	2	1	4	1	1
	----------------------------------------------------------------TCCCTCCCCCTCACTGCA----------------------	18	4	3	2	1	-	-	-	1	-	1
	---------------------------------------------------------------CTCCCTCCCCCTCACTGCACAGA------------------	23	-	-	-	-	-	-	-	-	1	2
	-----------------------------------------------------------------CCCTCCCCCTCACTGCACA--------------------	19	-	-	-	-	-	3	-	-	-	-
	----------------------------------------------------------------TCCCTCCCCCTCACTGCACAGAG-----------------	23	1	1	-	-	-	-	-	1	-	-
	---------------------------------------------------------------CTCCCTCCCCCTCACTGC-----------------------	18	-	-	-	-	-	-	-	1	1	-
	---------------------------------------------------------------CTCCCTCCCCCTCACTGCAC---------------------	20	-	1	-	-	-	-	-	-	-	-
rat	gctcaggactgcccagtgtgcagtggtgtgaggagggggcgtatcctaggcatcacactacctcTCCCTCCCCCTCACTGCACAGAgttgggctggtcacagcc
	********************************************************************************************************
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000027793 ENSRNOG00000020500 LOC365842
rat	(((...((((((((((((((((((((.....((((((((.......(((........)))...))))))))...))))))))))...)))))).))))..))).	1.000 -46.00

rat

chromosome:2:181255559:181255662:1

Same_strand|Boundary_coding|ENSRNOT00000027793|ENSRNOG00000020500 ## ENSRNOG00000020500|protein_coding|LOC365842|CDC-like kinase 2 [Source:RefSeq_peptide;Acc:NP_001014276]

block1791104_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1791104_novel 3arm	40.500	19	1	1	4	0.500	7.500	11.500	6	7.500
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1791104_novel 3arm	0.001	0.001	0.000	0.000	0.000	0.000	0.001	0.001	0.000	0.000

block1791104 [novel_cloningHIGH_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.002

no

no

0.42/0.50

18/23/0.78

0.4

0.2

37

0

10

0

15

4

3arm

1

0

0.29

3

101

10

na

na

		reads	miRBase family seed
seed	-----------------------------------------------------CUGACUU------------------------------	54	novel
seed	----------------------------------------------------CCUGACU-------------------------------	25	novel
seed	------------------------------------------------------UGACUUC-----------------------------	20	novel
seed	---------------------------------------------------ACCUGAC--------------------------------	2	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	----------------------------------------------------CCTGACTTCTCTCTTCATGCAG----------------	22	13	7	-	-	-	-	-	2	2	3
	----------------------------------------------------CCTGACTTCTCTCTTCATGCA-----------------	21	4	1	-	-	-	-	2	-	-	2
	-----------------------------------------------------CTGACTTCTCTCTTCATGCAG----------------	21	4	1	-	-	1	-	1	1	-	1
	---------------------------------------------------ACCTGACTTCTCTCTTCATGCAG----------------	23	4	-	-	-	1	-	-	2	1	-
	----------------------------------------------------CCTGACTTCTCTCTTCATG-------------------	19	1	2	-	-	1	-	1	2	-	-
	---------------------------------------------------ACCTGACTTCTCTCTTCATGCA-----------------	22	3	2	-	-	-	-	-	1	-	-
	-----------------------------------------------------CTGACTTCTCTCTTCATGCAGT---------------	22	5	-	-	-	-	-	-	1	-	-
	----------------------------------------------------CCTGACTTCTCTCTTCAT--------------------	18	1	-	-	-	-	1	1	1	-	1
	---------------------------------------------------ACCTGACTTCTCTCTTCA---------------------	18	-	3	-	-	1	-	-	-	-	1
	----------------------------------------------------CCTGACTTCTCTCTTCATGC------------------	20	-	2	-	-	-	-	1	-	1	-
	---------------------------------------------------ACCTGACTTCTCTCTTCATGC------------------	21	1	1	-	1	-	-	-	-	-	-
	-----------------------------------------------------CTGACTTCTCTCTTCATGCA-----------------	20	2	-	-	-	-	-	-	-	1	-
	-----------------------------------------------------CTGACTTCTCTCTTCATG-------------------	18	-	-	1	-	-	-	1	-	-	-
	----------------------------------------------------CCTGACTTCTCTCTTCATGCAGT---------------	23	1	-	-	-	-	-	-	1	-	-
	---------------------------------------------------ACCTGACTTCTCTCTTCAT--------------------	19	1	-	-	-	-	-	-	-	1	-
	--------------------------------------------------AACCTGACTTCTCTCTTCA---------------------	19	-	-	-	-	-	-	-	1	-	-
	---------------------------------------------------ACCTGACTTCTCTCTTCATG-------------------	20	-	-	-	-	-	-	1	-	-	-
	--------------------------------------------------AACCTGACTTCTCTCTTCATGC------------------	22	1	-	-	-	-	-	-	-	-	-
rat	cactaatggtgagcatgaagagttcagatcacgttcatgaagtgctgatgaACCTGACTTCTCTCTTCATGCAGTtgtctatcttggtgc
	******************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000017740 ENSRNOG00000013255 Dnajc8
rat	((((((.(((((((((((((((...((.(((.((((((.........)))))).)))))...)))))))))).....)).))))))))).	0.980 -29.60

rat

chromosome:5:152608900:152608989:-1

Same_strand|Boundary_coding|ENSRNOT00000017740|ENSRNOG00000013255 ## ENSRNOG00000013255|protein_coding|Dnajc8|DnaJ (Hsp40) homolog, subfamily C, member 8 Gene [Source:MGI (curated);Acc:Dnajc8-006]

Back to summary page

confident novel miRNAs:

Page 1

Previous page

Page 4

Next page

Page 6