miR-Intess™

confident novel miRNAs

novel_cloningHIGH_mirtron_randfoldOK (3 loci)

novel_cloningHIGH_randfoldOK (2 loci)

block2895936_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2895936_novel 3arm	2	7	1	2	0	1	1	0	158	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2895936_novel 3arm	0.000	0.000	0.000	0.000	0	0.000	0.000	0	0.010	0

block2895936 [novel_cloningHIGH_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.41/0.45

18/23/0.97

0.0

1.3

136

0

7

0

16

3

3arm

1

nd

0.17

2

172

7

na

na

		reads	miRBase family seed
seed	----------------------------------------------------------AAGAUGC-------------------------------	159	novel
seed	-----------------------------------------------------------AGAUGCC------------------------------	12	novel
seed	---------------------------------------------------------AAAGAUG--------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S2	T4S1	T5S1
	---------------------------------------------------------AAAGATGCCACGCTATGTAGATC----------------	23	-	5	-	-	-	-	62
	---------------------------------------------------------AAAGATGCCACGCTATGTAGA------------------	21	1	2	1	1	1	1	49
	---------------------------------------------------------AAAGATGCCACGCTATGTAG-------------------	20	-	-	-	-	-	-	25
	---------------------------------------------------------AAAGATGCCACGCTATGTAGAT-----------------	22	-	-	-	-	-	-	7
	----------------------------------------------------------AAGATGCCACGCTATGTAGAT-----------------	21	-	-	-	-	-	-	4
	----------------------------------------------------------AAGATGCCACGCTATGTAGATC----------------	22	-	-	-	-	-	-	4
	----------------------------------------------------------AAGATGCCACGCTATGTAGA------------------	20	1	-	-	-	-	-	2
	---------------------------------------------------------AAAGATGCCACGCTATGTA--------------------	19	-	-	-	1	-	-	1
	---------------------------------------------------------AAAGATGCCACGCTATGT---------------------	18	-	-	-	-	-	-	2
	----------------------------------------------------------AAGATGCCACGCTATGTA--------------------	18	-	-	-	-	-	-	1
	--------------------------------------------------------GAAAGATGCCACGCTATGTAGA------------------	22	-	-	-	-	-	-	1
rat	aatgtctcatgtgcattggtctacatagattggtacctatcatgtaaaataagtgtgAAAGATGCCACGCTATGTAGATCaacgctcatgacattc
	************************************************************************************************
rat	((((((..(((.((.(((((((((((((..(((((.((.((((...........)))).)).)))))..))))))))))))).)).))))))))).	1.000 -38.10

rat

chromosome:X:152842633:152842728:-1

intergenic

block735436_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block735436_novel 3arm	1	1.000	2	2	13	10.667	3.333	7.333	1	2
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block735436_novel 3arm	0.000	0.000	0.000	0.000	0.001	0.001	0.000	0.000	0.000	0.000

block735436 [novel_cloningHIGH_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.004

no

no

0.39/0.50

18/23/0.86

0.0

0.0

53

0

10

0

9

10

3arm

3

nd

0.13

2

130

10

na

na

Families

Member of family novel228 (seed AUACCAG): block735436_novel, block1125000_cand

		reads	miRBase family seed
seed	------------------------------------------------------------------AUACCAG------------------------	110	novel
seed	-------------------------------------------------------------------UACCAGG-----------------------	8	novel
seed	--------------------------------------------------------------------ACCAGGA----------------------	3	novel
seed	---------------------------------------------------------------------CCAGGAU---------------------	3	novel
seed	-----------------------------------------------------------------UAUACCA-------------------------	3	novel
seed	----------------------------------------------------------------CUAUACC--------------------------	2	novel
seed	----------------------------------------------------------------------CAGGAUG--------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	-----------------------------------------------------------------TATACCAGGATGTCAGCATAGTT---------	23	-	-	1	3	23	18	1	12	2	4
	-----------------------------------------------------------------TATACCAGGATGTCAGCATAGT----------	22	2	1	3	1	2	-	3	4	1	-
	-----------------------------------------------------------------TATACCAGGATGTCAGCATAG-----------	21	1	1	-	1	3	1	2	2	-	-
	-----------------------------------------------------------------TATACCAGGATGTCAGCA--------------	18	-	-	1	-	2	2	2	1	-	-
	-----------------------------------------------------------------TATACCAGGATGTCAGCATA------------	20	-	-	1	-	-	2	1	1	-	1
	------------------------------------------------------------------ATACCAGGATGTCAGCATAGTT---------	22	-	-	-	1	-	3	-	-	-	1
	-----------------------------------------------------------------TATACCAGGATGTCAGCAT-------------	19	-	-	-	-	2	1	-	1	-	-
	----------------------------------------------------------------CTATACCAGGATGTCAGC---------------	18	-	-	-	-	1	2	-	-	-	-
	------------------------------------------------------------------ATACCAGGATGTCAGCATAG-----------	20	-	1	-	-	1	-	-	-	-	-
	--------------------------------------------------------------------ACCAGGATGTCAGCATAGTT---------	20	-	-	-	-	1	1	-	-	-	-
	-------------------------------------------------------------------TACCAGGATGTCAGCATAGTT---------	21	-	-	-	-	1	-	1	-	-	-
	-------------------------------------------------------------------TACCAGGATGTCAGCATAGT----------	20	-	-	-	-	1	-	-	-	-	-
	---------------------------------------------------------------ACTATACCAGGATGTCAGCA--------------	20	-	-	-	-	1	-	-	-	-	-
	--------------------------------------------------------------------ACCAGGATGTCAGCATAGT----------	19	-	-	-	-	-	-	-	1	-	-
	---------------------------------------------------------------------CCAGGATGTCAGCATAGTT---------	19	-	-	-	-	-	1	-	-	-	-
	---------------------------------------------------------------ACTATACCAGGATGTCAGC---------------	19	-	-	-	-	-	1	-	-	-	-
	------------------------------------------------------------------ATACCAGGATGTCAGCATAGT----------	21	-	-	-	-	1	-	-	-	-	-
rat	gggagaaaagcagccaatagctggttggcattctgggcctggttcatgccaactctgttgttaacTATACCAGGATGTCAGCATAGTTgcttaccca
	*************************************************************************************************
rat	(((....(((((((...((....(((((((((((((...(((((.((..((....))..)).)))))..))))))))))))).))))))))).))).	0.770 -35.00

rat

chromosome:18:28178215:28178311:1

intergenic

novel_cloningOK_multiarm_DicerOK (2 loci)

sblock3286_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock3286_novel 5arm	5	1	0	0	3	0	3	8	2	4
sblock3286_novel 3arm	0	0	0	0	1	0	4	8	2	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock3286_novel 5arm	0.000	0.000	0	0	0.000	0	0.000	0.000	0.000	0.000
sblock3286_novel 3arm	0	0	0	0	0.000	0	0.000	0.000	0.000	0

sblock3286 [novel_cloningOK_multiarm_DicerOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.012

no

no

0.48/0.52

18/23/0.93

0.0 0.0

0.8 1.4

16 13

0 0

7 4

0 0

22 24

3 9

5arm 3arm

1 1

nd nd

0.14 0.24

1 2

41

7

3

3

		reads	miRBase family seed
seed	-----------------------GUCAGGA------------------------------------------------------------------------------	26	novel
seed	--------------------------------------------------------------GGGCACU---------------------------------------	14	novel
seed	-------------------------------------------------------------UGGGCAC----------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T3S1	T4S1	T4S2	T5S1	T5S2
	-------------------------------------------------------------TGGGCACTTATTTCCTGACAGAC------------------------	23	-	-	1	3	4	2	-
	----------------------AGTCAGGACATTGTGACCAG------------------------------------------------------------------	20	2	1	2	-	4	-	-
	----------------------AGTCAGGACATTGTGACCAGG-----------------------------------------------------------------	21	2	-	1	-	2	-	1
	----------------------AGTCAGGACATTGTGACCAGGA----------------------------------------------------------------	22	-	-	-	2	1	1	2
	-------------------------------------------------------------TGGGCACTTATTTCCTGACAG--------------------------	21	-	-	-	-	4	-	-
	----------------------AGTCAGGACATTGTGACCAGGAA---------------------------------------------------------------	23	-	-	-	1	1	1	-
	----------------------AGTCAGGACATTGTGACC--------------------------------------------------------------------	18	1	-	-	-	-	-	1
	------------------------------------------------------------CTGGGCACTTATTTCCTG------------------------------	18	-	-	-	1	-	-	-
rat	gccaccctgatctcatggggtcAGTCAGGACATTGTGACCAGGAagtactttgcatggttcTGGGCACTTATTTCCTGACAGACgccagaacagagtgctgggctggt
	************************************************************************************************************
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000001490 ENSRNOG00000001128 RGD1566317_predicted
rat	(((((((....(((.(((.(((.(((((((.((.(((.((((((.((.....))....)))))).)))..)).))))))).))).))).....)))....))).))))	0.990 -43.60

rat

chromosome:12:39675898:39676005:-1

Same_strand|Intronic_coding|ENSRNOT00000001490|ENSRNOG00000001128 ## ENSRNOG00000001128|protein_coding|RGD1566317_predicted|

sblock706_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock706_novel 5arm	0	0	0	0	0	1	0	0	3	0
sblock706_novel 3arm	0	3	1	0	0	0	1	0	22	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock706_novel 5arm	0	0	0	0	0	0.000	0	0	0.000	0
sblock706_novel 3arm	0	0.000	0.000	0	0	0	0.000	0	0.001	0

sblock706 [novel_cloningOK_multiarm_DicerOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.027

no

no

0.41/0.55

18/22/0.81

0.0 0.0 0.0

0.0 0.0 0.0

3 1 18

0 0 0

1 1 4

0 0 0

14 19 11

4 0 5

5arm 5arm 3arm

1 1 1

nd nd nd

0.30 0.32 0.32

3 3 4

31

5

1

2

Families

Member of family novel23 (seed CUCAGCA): sblock706_novel, block29776_cand, block1317251_cand, block97555_cand

		reads	miRBase family seed
seed	----------------------------------------------------------------CUCAGCA------------------------------	26	novel
seed	-------------------------------GCCUUGU---------------------------------------------------------------	3	novel
seed	-------------------------------------------------------------------AGCAAAG---------------------------	1	novel
seed	------------------------------------GUGAGAG----------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S2	T2S1	T3S2	T4S1	T5S1
	---------------------------------------------------------------TCTCAGCAAAGTATTGGGTAGA----------------	22	2	1	-	1	18
	------------------------------TGCCTTGTGAGAGCTGAGGA---------------------------------------------------	20	-	-	-	-	3
	---------------------------------------------------------------TCTCAGCAAAGTATTGGGT-------------------	19	-	-	-	-	3
	---------------------------------------------------------------TCTCAGCAAAGTATTGGG--------------------	18	1	-	-	-	-
	------------------------------------------------------------------CAGCAAAGTATTGGGTAGA----------------	19	-	-	-	-	1
	-----------------------------------TGTGAGAGCTGAGGATTTC-----------------------------------------------	19	-	-	1	-	-
rat	----------------CGGTCACTCCTCCCTGCCTTGTGAGAGCTGAGGATTTCAGCTGAGTATCTCAGCAAAGTATTGGGTAGATGGACAGTCCA-----
mouse	TCCTCACAACATGGGCTGCTCACTCCTCCCTGCCTTGTGAGAGCTGAGAACTTC-----AATATCTCAGCAATGTATCGGGTAGATGGACAGTCCAGAGGG
	* ***************************** * ***     * *********** **** ******************
rat	.((....(((...((((((.(((...(((((((..(((....)))..)))))))....))).))))))..)))....)).	0.960 -26.60
mouse	.((((......(((((((.....(((...((((((.(((...(((((((.....     ....)))))))....))).))))))..)))))))))))))).	0.970 -35.30

rat	chromosome:1:128997597:128997676:-1	intergenic
mouse	chromosome:7:80927688:80927783:-1	intergenic

novel_cloningOK_shortStem_mirtron_randfoldOK (2 loci)

block978236_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block978236_novel 3arm	6	1	0	1	0	0	0	0	3	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block978236_novel 3arm	0.000	0.000	0	0.000	0	0	0	0	0.000	0

block978236 [novel_cloningOK_shortStem_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.68/0.74

19/22/0.91

0.0

0.8

11

0

4

0

4

28

3arm

1

0

0.09

1

11

4

na

na

		reads	miRBase family seed
seed	------------------------------------------------------------------------------------------CCCCUCC------------------	11	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T5S1
	-----------------------------------------------------------------------------------------CCCCCTCCTCCCTCCTGACAGT----	22	3	1	-	2
	-----------------------------------------------------------------------------------------CCCCCTCCTCCCTCCTGACAG-----	21	1	-	-	1
	-----------------------------------------------------------------------------------------CCCCCTCCTCCCTCCTGACA------	20	2	-	-	-
	-----------------------------------------------------------------------------------------CCCCCTCCTCCCTCCTGAC-------	19	-	-	1	-
rat	ggtggctgtgaagatcaccccgaggagggggggggcaggaccctcgccgggggcaacatcctgtccccaaggcatgggtgactcctgacCCCCCTCCTCCCTCCTGACAGTaccc
	*******************************************************************************************************************
	>>>>>>>>>>>>+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>	ENSRNOT00000028290 ENSRNOG00000020848 ADCK4_RAT
	>>>>>>>>>>>>+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>	ENSRNOT00000057220 ENSRNOG00000020848 ADCK4_RAT
rat	(((.(((((..((........((((((((((((((((((((((..((((((((((......)))))))..)))..)))....))))).)))))))))))).)))).)))))))).	0.460 -65.60

rat

chromosome:1:82319032:82319146:1

Same_strand|Boundary_coding|ENSRNOT00000057220|ENSRNOG00000020848 ## ENSRNOG00000020848|protein_coding|ADCK4_RAT|Uncharacterized aarF domain-containing protein kinase 4 (EC 2.7.11.-). [Source:UniProtKB/Swiss-Prot;Acc:Q6AY19]

block1598968_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1598968_novel 3arm	3	3	1	8	5	1	1	2	2	1
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1598968_novel 3arm	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000

block1598968 [novel_cloningOK_shortStem_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.002

no

no

0.54/0.59

20/24/1.00

0.0

1.3

15

0

10

0

3

9

3arm

1

0

0.15

1

27

10

na

na

		reads	miRBase family seed
seed	----------------------------------------------------CAUUCCA------------------	22	novel
seed	--------------------------------------------------CUCAUUC--------------------	2	novel
seed	---------------------------------------------------UCAUUCC-------------------	2	novel
seed	-----------------------------------------------------AUUCCAC-----------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	---------------------------------------------------TCATTCCACCTTCCCTCCCAC-----	21	2	-	-	4	1	-	-	1	-	-
	---------------------------------------------------TCATTCCACCTTCCCTCCCACAG---	23	-	1	1	-	3	-	-	1	1	-
	---------------------------------------------------TCATTCCACCTTCCCTCCCA------	20	-	-	-	4	-	-	-	-	-	-
	---------------------------------------------------TCATTCCACCTTCCCTCCCACA----	22	-	2	-	-	1	-	-	-	-	-
	--------------------------------------------------CTCATTCCACCTTCCCTCCCACA----	23	1	-	-	-	-	-	-	-	-	-
	-------------------------------------------------TCTCATTCCACCTTCCCTCC--------	20	-	-	-	-	-	-	1	-	-	-
	--------------------------------------------------CTCATTCCACCTTCCCTCCCAC-----	22	-	-	-	-	-	-	-	-	1	-
	----------------------------------------------------CATTCCACCTTCCCTCCCACA----	21	-	-	-	-	-	1	-	-	-	-
	-------------------------------------------------TCTCATTCCACCTTCCCTCCCACA----	24	-	-	-	-	-	-	-	-	-	1
rat	accaatgggaggagggctgggaggaaaggagagagttctggaagcatcctcTCATTCCACCTTCCCTCCCACAGgtg
	*****************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>	ENSRNOT00000026184 ENSRNOG00000019264 Ltbr
rat	(((..(((((((((((.(((((.((.((((....(((.....))).)))).)).))))))))))).)))))..))).	1.000 -33.80

rat

chromosome:4:161432738:161432814:-1

Same_strand|Intronic_coding|ENSRNOT00000026184|ENSRNOG00000019264 ## ENSRNOG00000019264|protein_coding|Ltbr|lymphotoxin B receptor [Source:RefSeq_peptide;Acc:NP_001008316]

miRNAunknown_cloningOK_randfoldOK (1 loci)

block2895963_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2895963_novel 3arm	1	2	0	1	0	1	0	0	10	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2895963_novel 3arm	0.000	0.000	0	0.000	0	0.000	0	0	0.001	0

block2895963 (miRNA) [miRNAunknown_cloningOK_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

Ensembl miRNA

0.004

no

no

0.43/0.47

18/24/0.73

0.6

1.5

15

0

5

0

18

3

3arm

1

nd

0.16

2

15

5

na

na

		reads	miRBase family seed
seed	--------------------------------------------------------GAUCAGU----------------------------------	12	novel
seed	------------------------------------------------------------AGUGCCU------------------------------	2	novel
seed	---------------------------------------------------------AUCAGUG---------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T3S2	T5S1
	-------------------------------------------------------TGATCAGTGCCTTTCTGAGTAGA-------------------	23	-	1	-	-	3
	-------------------------------------------------------TGATCAGTGCCTTTCTGAGT----------------------	20	1	-	-	-	2
	-------------------------------------------------------TGATCAGTGCCTTTCTGAGTAG--------------------	22	-	-	-	1	1
	-----------------------------------------------------------CAGTGCCTTTCTGAGTAGA-------------------	19	-	-	-	-	2
	-------------------------------------------------------TGATCAGTGCCTTTCTGAGTAGAG------------------	24	-	-	-	-	1
	--------------------------------------------------------GATCAGTGCCTTTCTGAGT----------------------	19	-	1	-	-	-
	-------------------------------------------------------TGATCAGTGCCTTTCTGA------------------------	18	-	-	-	-	1
	-------------------------------------------------------TGATCAGTGCCTTTCTGAGTA---------------------	21	-	-	1	-	-
rat	aagggcttggatgcagtgccctatttagaacggtgctggtgtgatgaatgaagttTGATCAGTGCCTTTCTGAGTAGAGtaaggcttaccacattct
	*************************************************************************************************
	................>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>..............	ENSRNOT00000062157 ENSRNOG00000040404
rat	.((((..(((..((..(((.((((((((((.((..(((((..(((.......)))..)))))..)).)))))))))).)))..))...)))..))))	1.000 -34.90

rat

chromosome:X:152868872:152868968:-1

Same_strand|Exonic_non-coding|ENSRNOT00000062157|ENSRNOG00000040404 ## ENSRNOG00000040404|miRNA||

novel_cloningHIGH_multiarm_DicerOK_mirtron (1 loci)

sblock5255_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock5255_novel 5arm	0	1	0	0	0	0	0	0	0	0
sblock5255_novel 3arm	23	10.500	0	2	7	5	7.500	8	7	5.500
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock5255_novel 5arm	0	0.000	0	0	0	0	0	0	0	0
sblock5255_novel 3arm	0.001	0.000	0	0.000	0.000	0.000	0.001	0.000	0.000	0.000

sblock5255 [novel_cloningHIGH_multiarm_DicerOK_mirtron]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.022

no

no

0.44/0.60

18/22/0.81

0.0 0.0

0.0 0.9

1 51

0 0

1 9

0 0

22 12

14 18

5arm 3arm

1 1

1 0

0.10 0.10

1 1

79

9

1

3

Families

Member of family novel177 (seed AGGCUCU): sblock5255_novel, sblock2920_cand

		reads	miRBase family seed
seed	------------------------------------------------------------------------------------AGGCUCU--------------------------	78	novel
seed	-----------------------UAGGGAG---------------------------------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	-----------------------------------------------------------------------------------AAGGCTCTTCTTTCCTTGCAGT------------	22	8	1	-	5	1	5	4	4	2
	-----------------------------------------------------------------------------------AAGGCTCTTCTTTCCTTGCA--------------	20	7	2	1	2	1	-	2	2	-
	-----------------------------------------------------------------------------------AAGGCTCTTCTTTCCTTGCAG-------------	21	4	3	1	-	2	1	1	1	3
	-----------------------------------------------------------------------------------AAGGCTCTTCTTTCCTTGC---------------	19	4	4	-	-	1	1	-	-	-
	-----------------------------------------------------------------------------------AAGGCTCTTCTTTCCTTG----------------	18	-	1	-	-	-	1	2	-	1
	----------------------GTAGGGAGAGTGGGGCATTG---------------------------------------------------------------------------	20	-	1	-	-	-	-	-	-	-
rat	cctgaagaaatcgagaactatggtagggagagtggggcattggagctgcacagaggagggtcaggcctctgggtggtagaaccAAGGCTCTTCTTTCCTTGCAGTgcaccgaatcgg
	*********************************************************************************************************************
	>>>>>>>>>>>>>>>>>>>>>>>>>>>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>	ENSRNOT00000018114 ENSRNOG00000012771 LOC688806
rat	.((((.....(((...(((...((((((((((.(((((.((((..((((.((((((.........))))))....))))..)))).))))).)))))))))))))....))).))))	0.930 -44.30

rat

chromosome:17:15151552:15151668:1

Same_strand|Boundary_coding|ENSRNOT00000018114|ENSRNOG00000012771 ## ENSRNOG00000012771|protein_coding|LOC688806|DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 [Source:RefSeq_peptide;Acc:NP_001101516]

novel_cloningOK_multiarm_DicerOK_randfoldOK (1 loci)

sblock10893_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock10893_novel 5arm	0	2	0	0	0	0	0	0	31	0
sblock10893_novel 3arm	1	0	0	0	0	0	0	0	1	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock10893_novel 5arm	0	0.000	0	0	0	0	0	0	0.002	0
sblock10893_novel 3arm	0.000	0	0	0	0	0	0	0	0.000	0

sblock10893 [novel_cloningOK_multiarm_DicerOK_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.004

no

no

0.36/0.54

18/26/0.94

0.3 5.0

0.0 2.0

23 2

0 0

2 2

0 0

10 2

11 15

5arm 3arm

1 1

nd nd

0.27 0.15

3 3

35

3

2

2

Families

Member of family novel49 (seed CCCCAGA): sblock10893_novel, block1559688_cand, block1768036_cand

		reads	miRBase family seed
seed	------------GCUAUUU----------------------------------------------------------------------------	21	novel
seed	-----------UGCUAUU-----------------------------------------------------------------------------	11	novel
seed	----------------------------------------------------------------CCCCAGA------------------------	1	novel
seed	-------------CUAUUUC---------------------------------------------------------------------------	1	novel
seed	--------------------------------------------------------------------------AGAAAAG--------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T5S1
	-----------TGCTATTTCTTAATCCGGAGA---------------------------------------------------------------	21	-	2	15
	----------CTGCTATTTCTTAATCCGGAGA---------------------------------------------------------------	22	-	-	8
	-----------TGCTATTTCTTAATCCGGAGAA--------------------------------------------------------------	22	-	-	2
	-----------TGCTATTTCTTAATCCGGAG----------------------------------------------------------------	20	-	-	2
	----------CTGCTATTTCTTAATCCGGAG----------------------------------------------------------------	21	-	-	2
	---------------------------------------------------------------TCCCCAGAGGTAGAAAAGCAGCAGAG------	26	-	-	1
	------------GCTATTTCTTAATCCGGAGA---------------------------------------------------------------	20	-	-	1
	-------------------------------------------------------------------------TAGAAAAGCAGCAGAGCTTG--	20	1	-	-
	----------CTGCTATTTCTTAATCCG-------------------------------------------------------------------	18	-	-	1
rat	tggcaagtctCTGCTATTTCTTAATCCGGAGAatttgaggtagaagagctgggtcacaaagtctccccagaggtagaaaagcagcagagcttgct
	***********************************************************************************************
rat	.((((((.(((((((.(((((...((.(((((.((((.(((((.....)))..)).)))).)))))...))...)))))...)))))))))))))	0.980 -32.90

rat

chromosome:7:96046923:96047017:-1

intergenic

novel_multiarm_DicerOK_mirtron_randfoldOK (1 loci)

sblock2545_novel

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock2545_novel 5arm	1	0	0	0	0	0	0	0	0	0
sblock2545_novel 3arm	1	0	0	0	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock2545_novel 5arm	0.000	0	0	0	0	0	0	0	0	0
sblock2545_novel 3arm	0.000	0	0	0	0	0	0	0	0	0

sblock2545 [novel_multiarm_DicerOK_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.002

no

no

0.59/0.64

22/22/1.00

0.0 0.0

0.0 0.0

1 1

0 0

1 1

0 0

25 22

4 9

5arm 3arm

1 1

44 1

0.23 0.18

1 1

2

1

2

2

Families

Member of family miR-130a/130b/301a/301b (seed AGUGCAA): rno-mir-301a, rno-mir-130a, rno-mir-301b, rno-mir-130b, sblock2545_novel

		reads	miRBase family seed
seed	--------------------------UGGGGCA-------------------------------------------------------------------------------	1	novel
seed	---------------------------------------------------------------------AGUGCAA------------------------------------	1	miR-130a/130b/301a/301b
		len	cloning frequencies
			T1S1
	--------------------------------------------------------------------TAGTGCAACCCCTGTCCCCACA----------------------	22	1
	-------------------------TTGGGGCAGTGGCTGGATGGGA-----------------------------------------------------------------	22	1
rat	tctggtatatcaggcttgctgtgatTTGGGGCAGTGGCTGGATGGGAggggactcacagccctatctcTAGTGCAACCCCTGTCCCCACAggtctagagcctgagatccaga
	****************************************************************************************************************
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000005977 ENSRNOG00000004430 NP_001101780.1
rat	(((((....((((((((.(((((....(((((((.((.((.((.(((((((........)))...)))).)).)).)).)))))))))))).....))))))))...)))))	0.960 -50.80

rat

chromosome:10:109478589:109478700:-1

Same_strand|Intronic_coding|ENSRNOT00000005977|ENSRNOG00000004430 ## ENSRNOG00000004430|protein_coding|NP_001101780.1|5-azacytidine induced gene 1 [Source:RefSeq_peptide;Acc:NP_001101780]

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confident novel miRNAs:

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