miR-Intess™

candidate novel miRNAs

novel_mirtron_randfoldOK (60 loci)

block2355811_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2355811_cand 3arm	0	0	0	0	1	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2355811_cand 3arm	0	0	0	0	0.000	0	0	0	0	0

block2355811 [novel_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.60/0.60

20/20/1.00

0.0

0.0

1

0

1

0

24

14

3arm

1

2

0.20

2

1

1

na

na

Families

Member of family novel181 (seed UCCCUCC): block2355811_cand, block2796013_cand

		reads	miRBase family seed
seed	------------------------------------------------------------------------------------------------UCCCUCC--------------------------------------------------------	1	novel
		len	cloning frequencies
			T3S1
	-----------------------------------------------------------------------------------------------GTCCCTCCCTGCATGTAGAC--------------------------------------------	20	1
rat	-------------------GGATGGTGAGGCGCCTGGCTGGTAAGCAGGCAGGGTGGGAAGAAGGGGCAGCCATAGCTTTGGGCTCCCCCTAATCGTCCCTCCCTGCATGTAGACTGCTTTGGCTGCCTTTCCTGGTCA--------------------
mouse	AGCATGGGCCTGTGTATGAAGATGGTGAGGCGCCTGGCTGGTAAGCAGGAGGGGTGGGCAGAAGGGGCAGCTATAGCATTGGGCTCCCCCTAACCGTCCCTCCCTGCATGCAGACTGCTTTGGCTGCCTCTCCTGGTCACACTCAGAGACGCACTTGTT
	*****************************  ******* ************ ***** *************** **************** ****************** *********
	>>>>>                   >>>>>+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>                    >>>>>	ENSRNOT00000045410 ENSRNOG00000029572 Apeh
rat	.(((((.((((((((.(((.(((..(((.((((((.((((.(((((((.((((.........)))).)))))..)).)))).)))))).)))..))))))..))).))))).))..))).	1.000 -62.70
mouse	((((...(((((.((.(((....((.((((((((.(((..(((.(((((((((..((..((..((((.((((.........))))))))))..))..)))))).))).))).....)))..))).))))).))...))).)).)))....))...))))	0.987 -69.00

rat	chromosome:8:113360342:113360461:-1	Same_strand|Boundary_coding|ENSRNOT00000045410|ENSRNOG00000029572 ## ENSRNOG00000029572|protein_coding|Apeh|Acylamino-acid-releasing enzyme (EC 3.4.19.1) (AARE) (Acyl-peptide hydrolase) (APH) (Acylaminoacyl-peptidase). [Source:UniProtKB/Swiss-Prot;Acc:P13676]
mouse	chromosome:9:107995003:107995161:-1	Same_strand|Boundary_coding|ENSMUST00000081309|ENSMUSG00000032590|protein_coding|acylpeptide hydrolase Gene [Source:MGI Symbol;Acc:MGI:88041]

block2357765_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2357765_cand 5arm	0	2	0	0	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2357765_cand 5arm	0	0.000	0	0	0	0	0	0	0	0

block2357765 [novel_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.003

no

no

0.57/0.60

20/21/1.00

0.0

0.5

2

0

1

0

8

26

5arm

1

1

0.24

3

2

1

na

na

		reads	miRBase family seed
seed	---------CAGCCGA-------------------------------------------------------------------------------------------	2	novel
		len	cloning frequencies
			T1S2
	--------TCAGCCGACTCCTTTCCCTCA------------------------------------------------------------------------------	21	1
	--------TCAGCCGACTCCTTTCCCTC-------------------------------------------------------------------------------	20	1
rat	ctgacatcTCAGCCGACTCCTTTCCCTCAggtccccagttgggccatggatatgaatgtgctcatgtctgcccagctgctgatggagcaggtcgctgctgatagcag
	***********************************************************************************************************
	+++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000056130 ENSRNOG00000037181 AC158139.11
rat	(((..(((.(((.(((((...((((.((((.....(((((((((...((((((((......))))))))))))))))))))).))))..))))))))..)))..)))	1.000 -45.80

rat

chromosome:8:115016621:115016727:-1

Same_strand|Intronic_coding|ENSRNOT00000056130|ENSRNOG00000037181 ## Same_strand|Boundary_non-coding|ENSRNOT00000056129|ENSRNOG00000037181 ## ENSRNOG00000037181|protein_coding|AC158139.11|neurobeachin like 1 [Source:RefSeq peptide;Acc:NP_775620]

block2450448_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2450448_cand 3arm	0	1	1	0	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2450448_cand 3arm	0	0.000	0.000	0	0	0	0	0	0	0

block2450448 [novel_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.62/0.65

20/21/1.00

0.5

0.0

2

0

2

0

15

5

3arm

1

0

0.19

3

2

2

na

na

Families

Member of family novel67 (seed CUCUGCC): block757922_cand, block2450448_cand

		reads	miRBase family seed
seed	--------------------------------------------------------CUCUGCC----------------------------	1	novel
seed	---------------------------------------------------------UCUGCCC---------------------------	1	novel
		len	cloning frequencies
			T1S2	T2S1
	-------------------------------------------------------ACTCTGCCCTCCCTTGACCAG---------------	21	-	1
	--------------------------------------------------------CTCTGCCCTCCCTTGACCAG---------------	20	1	-
rat	gggactggacccaactacttcaagtgggggtcaggaagtttgagatatgccttgaACTCTGCCCTCCCTTGACCAGgtttccccagaccca
	*******************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000021408 ENSRNOG00000015858 Hyal1
rat	(((.((((....((((...(((((.(((((.(((..((((..((......))..)))))))))))).)))))...))))...)))).))).	1.000 -37.40

rat

chromosome:8:112824985:112825075:1

Same_strand|Intronic_coding|ENSRNOT00000021408|ENSRNOG00000015858 ## ENSRNOG00000015858|protein_coding|Hyal1|Hyaluronidase-1 precursor (EC 3.2.1.35) (Hyal-1) (Hyaluronoglucosaminidase-1). [Source:UniProtKB/Swiss-Prot;Acc:Q76HN1]

block2567348_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2567348_cand 3arm	1	0	0	0	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2567348_cand 3arm	0.000	0	0	0	0	0	0	0	0	0

block2567348 [novel_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.001

no

no

0.59/0.59

22/22/1.00

0.0

0.0

1

0

1

0

25

12

3arm

1

0

0.18

2

1

1

na

na

		reads	miRBase family seed
seed	--------------------------------------------------------------------UCUCCAU---------------------------------------	1	novel
		len	cloning frequencies
			T1S1
	-------------------------------------------------------------------CTCTCCATCCCCTTCCTTCCAG-------------------------	22	1
rat	aggagcaggtgagtagccaccagtgtggagttaggggtgggaccctggtggcactgccgcctcttcaCTCTCCATCCCCTTCCTTCCAGgtgaacaagtttctcacctcatcct
	******************************************************************************************************************
	----------------------------------------------------------------------------------------------------------------------------	ENSRNOT00000025335 ENSRNOG00000018741 LOC683524
	>>>>>>>>>>>>>+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000024266 ENSRNOG00000017857 LOC682940
	>>>>>>>>>>>>>+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000061305 ENSRNOG00000017857 LOC682940
rat	((((..(((((((.(((((((..((.((((..((((((((((....((((((...)))))).......)).))).)))))..)))))))))).....))).)))))))..))))	1.000 -49.60

rat

chromosome:9:10029894:10030007:1

Opposite_strand|Intronic_coding|ENSRNOT00000025335|ENSRNOG00000018741 ## Same_strand|Intronic_coding|ENSRNOT00000024266|ENSRNOG00000017857 ## ENSRNOG00000018741|protein_coding|LOC683524| ## ENSRNOG00000017857|protein_coding|LOC682940|

block2737854_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2737854_cand 3arm	0	0	0	0.333	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2737854_cand 3arm	0	0	0	0.000	0	0	0	0	0	0

block2737854 [novel_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.008

no

no

0.60/0.60

20/20/1.00

0.0

0.0

1

0

1

0

9

21

3arm

3

2

0.15

3

1

1

na

na

		reads	miRBase family seed
seed	--------------------------------------------------------------------------------------------AAGCCUG---------------------	1	novel
		len	cloning frequencies
			T2S2
	-------------------------------------------------------------------------------------------AAAGCCTGAGCAGGTGGAGC---------	20	1
rat	aggacccagttgatctccaacctgaagaaagagggcttgttccacaactggcagcacatcaccgaccagattggcatgttctgcttcacttAAAGCCTGAGCAGGTGGAGCgactggcca
	************************************************************************************************************************
	>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000044402 ENSRNOG00000032385
	>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000050240 ENSRNOG00000032385
rat	.((..(((((((..((((.(((((........((((((...........(((((.((((..((((.....)))).)))).))))).......))))))...)))))))))))))))))).	0.800 -42.77

rat

chromosome:X:83244688:83244807:-1

Same_strand|Boundary_coding|ENSRNOT00000050240|ENSRNOG00000032385 ## ENSRNOG00000032385|protein_coding||

novel_cloningOK (51 loci)

block115001_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block115001_cand 3arm	7.500	0	1	0	2	0	0	1	1	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block115001_cand 3arm	0.000	0	0.000	0	0.000	0	0	0.000	0.000	0

block115001 [novel_cloningOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.017

no

no

0.48/0.67

18/24/0.92

0.0

0.0

8

0

5

0

6

32

3arm

1

nd

0.29

2

13

5

na

na

Families

Member of family novel14 (seed UUUUUGG): block115001_cand, block1369713_cand, block1404988_cand, block1426367_cand, block2490925_cand, block2730600_cand

		reads	miRBase family seed
seed	--------------------------------------------------------------------------------------------UUUUUGG-------------------	12	novel
seed	---------------------------------------------------------------------------------------------------GGGGUGG------------	1	novel
		len	cloning frequencies
			T1S1	T2S1	T3S1	T4S2	T5S1
	-------------------------------------------------------------------------------------------TTTTTTGGGGGGTGGGGTTTT------	21	6	1	2	-	1
	--------------------------------------------------------------------------------------------------GGGGGTGGGGTTTTGGTG--	18	-	-	-	1	-
	-------------------------------------------------------------------------------------------TTTTTTGGGGGGTGGGGTTTTGGT---	24	1	-	-	-	-
	-------------------------------------------------------------------------------------------TTTTTTGGGGGGTGGGGTTT-------	20	1	-	-	-	-
rat	gatcaatgggcctctgatttttaagaagctataactaaactctcaggtgagttttttgtaagattaataggagagttgcaaatcatctgggTTTTTTGGGGGGTGGGGTTTTggtgat
	**********************************************************************************************************************
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000049121 ENSRNOG00000006997 App
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000042130 ENSRNOG00000006997 App
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000048854 ENSRNOG00000006997 App
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000044081 ENSRNOG00000006997 App
rat	.((((..(((((((...((..(((((((((.......(((((((..((.((((((....)))))).))..)))))))............)))))))))..))..)))))))...))))	0.910 -31.61

rat

chromosome:11:24480138:24480255:-1

Same_strand|Intronic_coding|ENSRNOT00000049121|ENSRNOG00000006997 ## ENSRNOG00000006997|protein_coding|App|Amyloid beta A4 protein precursor (APP) (ABPP) (Alzheimer disease amyloid protein homolog) (Amyloidogenic glycoprotein) (AG) [Contains: Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99; Beta-amyloid protein 42 (Beta-APP42); Beta-amyloid [Source:UniProtKB/Swiss-Prot;Acc:P08592]

block174914_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block174914_cand 3arm	0	0	1	0	0	0	0	0	13	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block174914_cand 3arm	0	0	0.000	0	0	0	0	0	0.001	0

block174914 [novel_cloningOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.012

no

no

0.40/0.45

19/25/0.79

0.0 0.3

0.0 1.3

9 3

0 0

2 1

0 0

41 29

0 8

3arm 3arm

1 1

nd nd

0.33 0.20

6 2

14

2

na

na

		reads	miRBase family seed
seed	-----------------------------------------------------------GACCAAA------------------------------------------------------	10	novel
seed	-------------------------------------------------------------------GACCUAU----------------------------------------------	2	novel
seed	--------------------------------------------------------CCUGACC---------------------------------------------------------	1	novel
seed	--------------------------------------------------------------------ACCUAUG---------------------------------------------	1	novel
		len	cloning frequencies
			T2S1	T5S1
	----------------------------------------------------------TGACCAAATGACCTATGCTGT-----------------------------------------	21	1	9
	------------------------------------------------------------------TGACCTATGCTGTTTTCCAGATATG-----------------------------	25	-	2
	-------------------------------------------------------------------GACCTATGCTGTTTTCCAGA---------------------------------	20	-	1
	-------------------------------------------------------TCCTGACCAAATGACCTAT----------------------------------------------	19	-	1
rat	cctgtgccaagagaagggcaatggctggcccagagatggcaaaggcctgggtcaatccTGACCAAATGACCTATGCTGTtttccagatatgcagaccacatcctgtctctttttcacagt
	************************************************************************************************************************
rat	.(((((..((((((.(((...(((((((....(((((((((.(((....(((((....)))))......))).))))))))))))).........)))...))).))))))...))))).	0.760 -39.40

rat

chromosome:11:84966347:84966466:1

intergenic

block333328_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block333328_cand 3arm	5	5	6	3	5	4	3	1	1	3
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block333328_cand 3arm	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000

block333328 [novel_cloningOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.034

no

no

0.73/0.80

18/23/0.75

0.0

1.5

36

0

10

0

31

2

3arm

1

nd

0.05

1

36

10

na

na

		reads	miRBase family seed
seed	-----------------------------------------------------------------CGAGCCU----------------------------------------------	35	novel
seed	------------------------------------------------------------------GAGCCUC---------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	----------------------------------------------------------------TCGAGCCTCGCGTCCGCCGAC---------------------------------	21	-	-	-	2	2	2	1	1	-	1
	----------------------------------------------------------------TCGAGCCTCGCGTCCGCCGACAC-------------------------------	23	2	1	1	-	2	1	-	-	-	1
	----------------------------------------------------------------TCGAGCCTCGCGTCCGCC------------------------------------	18	1	1	1	1	-	1	2	-	1	-
	----------------------------------------------------------------TCGAGCCTCGCGTCCGCCGA----------------------------------	20	-	2	3	-	1	-	-	-	-	1
	----------------------------------------------------------------TCGAGCCTCGCGTCCGCCGACA--------------------------------	22	1	-	1	-	-	-	-	-	-	-
	----------------------------------------------------------------TCGAGCCTCGCGTCCGCCG-----------------------------------	19	1	-	-	-	-	-	-	-	-	-
	-----------------------------------------------------------------CGAGCCTCGCGTCCGCCGAC---------------------------------	20	-	1	-	-	-	-	-	-	-	-
rat	GCCCCCACGGCCAGCCGTGGCGGTTGTCAAGGCGCGGGCGCGGGGCTCCGCCATAAAGGGGGGGTCGAGCCTCGCGTCCGCCGACACAAATCCGCCTGCGCCTCCGTGCTTAAAGGGG
mouse	------------------------CGTCAAGGCGCGGGCGCGGGGCTCCGCCATAAA-GGGGAGTCGAGCCTCGCGTCCGCCGACA--------------------------------
	******************************** **** ***********************
rat	.(((((((((..((.((((((((.((((.....(((((((((((((((.(((..........))).))))))))))))))).)))).....))))).))).)))))))......))))	0.860 -63.40
mouse	.(((.....(((((((((((((((..((..... ..))....))))))))))))))).))).	0.994 -35.60

rat	chromosome:14:2325995:2326112:-1	intergenic ## {SimpF: 1 Eponine, -1 Eponine}
mouse	chromosome:5:108416179:108416336:1	intergenic ## {Repeats: dust 0 class=dust} ## {SimpF: oe = 0.96 1 CpG, -1 Eponine, -1 Eponine, 1 Eponine, -1 Eponine, 1 Eponine, -1 Eponine}

block361641_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block361641_cand 3arm	1	0	3	0	1	1	2	0	18	2
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block361641_cand 3arm	0.000	0	0.000	0	0.000	0.000	0.000	0	0.001	0.000

block361641 [novel_cloningOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.836

no

no

0.32/0.35

19/20/0.75

0.0

0.0

14

0

7

0

18

1

3arm

1

nd

0.30

2

28

7

na

na

		reads	miRBase family seed
seed	-------------------------------------------------------------UUAAUGA------------------------------	28	novel
		len	cloning frequencies
			T1S1	T2S1	T3S1	T3S2	T4S1	T5S1	T5S2
	------------------------------------------------------------TTTAATGATGACTTCTGGTG------------------	20	1	2	1	1	1	14	1
	------------------------------------------------------------TTTAATGATGACTTCTGGT-------------------	19	-	1	-	-	1	4	1
rat	gaggctttcccttatgttgctaactgaaggaatttcttaaagaattgaagacccccccccTTTAATGATGACTTCTGGTGccaattcacaatgctttt
	**************************************************************************************************
rat	(((((..........((((...(((((((..(((..((((((.................)))))).)))..)))).)))..)))).......))))).	0.600 -13.56

rat

chromosome:14:71291989:71292086:-1

intergenic

block379175_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block379175_cand 5arm	4	5	3	2	0	3	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block379175_cand 5arm	0.000	0.000	0.000	0.000	0	0.000	0	0	0	0

block379175 [novel_cloningOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.100

no

no

0.55/0.61

18/25/0.82

0.6

1.2

17

0

5

0

31

0

5arm

1

25

0.20

2

17

5

na

na

Families

Member of family novel121 (seed UGCGGGA): block270016_cand, block379175_cand

		reads	miRBase family seed
seed	---------------------------------GCGGGAA-------------------------------------------------------------	9	novel
seed	--------------------------------UGCGGGA--------------------------------------------------------------	7	novel
seed	----------------------------------CGGGAAG------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S2
	-------------------------------TTGCGGGAAGGGTAGCATGAGT------------------------------------------------	22	3	1	2	-	-
	--------------------------------TGCGGGAAGGGTAGCATGAG-------------------------------------------------	20	-	1	1	-	1
	--------------------------------TGCGGGAAGGGTAGCATGAGT------------------------------------------------	21	1	-	-	1	-
	--------------------------------TGCGGGAAGGGTAGCATGAGTACC---------------------------------------------	24	-	1	-	1	-
	--------------------------------TGCGGGAAGGGTAGCATG---------------------------------------------------	18	-	-	-	-	1
	--------------------------------TGCGGGAAGGGTAGCATGA--------------------------------------------------	19	-	1	-	-	-
	-------------------------------TTGCGGGAAGGGTAGCATGAGTACC---------------------------------------------	25	-	-	-	-	1
	---------------------------------GCGGGAAGGGTAGCATGAGTACC---------------------------------------------	23	-	1	-	-	-
rat	ggctcaagtaagactcaaagcacaggatctgTTGCGGGAAGGGTAGCATGAGTACCtagaacctggactctgagcctcttccccacagggtaggtgtggcc
	*****************************************************************************************************
	>>>>>>>>>>>>+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>	ENSRNOT00000032056 ENSRNOG00000024112 Dgkq
rat	((((...............((((....((((.((.(((((((((..((.((((.((........)))))))).))).))))))))))))....))))))))	0.440 -34.46

rat

chromosome:14:1606526:1606626:1

Same_strand|Intronic_coding|ENSRNOT00000032056|ENSRNOG00000024112 ## ENSRNOG00000024112|protein_coding|Dgkq|diacylglycerol kinase, theta Gene [Source:MGI (curated);Acc:Dgkq-001]

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candidate novel miRNAs:

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