miR-Intess™

candidate novel miRNAs

novel_lenNOK_cloningOK_mirtron_randfoldOK (5 loci)

block1091923_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1091923_cand 3arm	0	0	1	4	3	4	0	0	2	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block1091923_cand 3arm	0	0	0.000	0.000	0.000	0.000	0	0	0.000	0

block1091923 [novel_lenNOK_cloningOK_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.004

no

no

0.44/0.52

18/25/0.43

0.0

3.2

14

0

5

0

17

9

3arm

1

0

0.15

2

14

5

na

na

		reads	miRBase family seed
seed	-----------------------------------------------------------------UACUUUC----------------------------------	14	novel
		len	cloning frequencies
			T2S1	T2S2	T3S1	T3S2	T5S1
	----------------------------------------------------------------CTACTTTCTTGCCCTCTA------------------------	18	-	-	-	3	-
	----------------------------------------------------------------CTACTTTCTTGCCCTCTACCAGAC------------------	24	1	-	2	-	-
	----------------------------------------------------------------CTACTTTCTTGCCCTCTAC-----------------------	19	-	3	-	-	-
	----------------------------------------------------------------CTACTTTCTTGCCCTCTACCAGACC-----------------	25	-	-	-	-	2
	----------------------------------------------------------------CTACTTTCTTGCCCTCTACCAG--------------------	22	-	1	1	-	-
	----------------------------------------------------------------CTACTTTCTTGCCCTCTACC----------------------	20	-	-	-	1	-
rat	ctccagggcaaaaggctgggctgagggatggggaaggaggcaagatgtactccctgtagcttgaCTACTTTCTTGCCCTCTACCAGACCtgagctgtcccaaggag
	**********************************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000000514 ENSRNOG00000000443
rat	((((.(((((..(((((((...(((((.((((((((.((.((((...(((.....))).)))).)).)))))))))))))..)))).)))....)))))...))))	0.990 -44.40

rat

chromosome:20:4111876:4111981:1

Same_strand|Boundary_coding|ENSRNOT00000000514|ENSRNOG00000000443 ## ENSRNOG00000000443|protein_coding||

block2184179_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2184179_cand 3arm	5	2	1	2	2	0	1	4	0	1
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block2184179_cand 3arm	0.000	0.000	0.000	0.000	0.000	0	0.000	0.000	0	0.000

block2184179 [novel_lenNOK_cloningOK_mirtron_randfoldOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.010

no

no

0.53/0.56

18/20/0.39

0.0

0.7

18

0

8

0

9

26

3arm

1

0

0.15

1

18

8

na

na

		reads	miRBase family seed
seed	-----------------------------------------------------------------------------------GACAGCU---------------------	18	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T4S1	T4S2	T5S2
	----------------------------------------------------------------------------------TGACAGCTCCTCTCCTACAG---------	20	-	-	1	-	2	1	2	1
	----------------------------------------------------------------------------------TGACAGCTCCTCTCCTACA----------	19	3	2	-	-	-	-	1	-
	----------------------------------------------------------------------------------TGACAGCTCCTCTCCTAC-----------	18	2	-	-	2	-	-	1	-
rat	gtcctagccagtaggggttggagctgtggccccagttggagccctgggaggtcttggcaggggggctttgagctcagggtggTGACAGCTCCTCTCCTACAGcggaagacc
	***************************************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>	ENSRNOT00000039232 ENSRNOG00000024879 NP_001100236.1
rat	(((...((..(((((((..((((((((.(((((((((((((((((.....((....))...)))))))).))))...)).))).)))))))).))))))).))....))).	0.470 -53.40

rat

chromosome:7:11479692:11479802:1

Same_strand|Intronic_coding|ENSRNOT00000039232|ENSRNOG00000024879 ## ENSRNOG00000024879|protein_coding|NP_001100236.1|polymerase (RNA) mitochondrial (DNA directed) [Source:RefSeq_peptide;Acc:NP_001100236]

novel_lenNOK_cloningOK_multiarm_DicerOK (5 loci)

sblock12668_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12668_cand 5arm	9	6	0	0	0	0	4	10	0	0
sblock12668_cand 3arm	0	0	0	0	0	0	0	1	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock12668_cand 5arm	0.000	0.000	0	0	0	0	0.000	0.001	0	0
sblock12668_cand 3arm	0	0	0	0	0	0	0	0.000	0	0

sblock12668 [novel_lenNOK_cloningOK_multiarm_DicerOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.061

no

no

0.48/0.61

18/21/0.20

0.0 0.0

0.0 0.0

22 1

0 0

4 1

0 0

24 26

10 10

5arm 3arm

1 1

nd nd

0.33 0.28

2 2

30

4

2

2

Families

Member of family novel120 (seed CUGGCUC): block220608_cand, sblock12668_cand

		reads	miRBase family seed
seed	-------------------------CUGGCUC-------------------------------------------------------------------------------	28	novel
seed	--------------------------------------------------------------------AGGUACA------------------------------------	1	novel
seed	-----------------------UUCUGGC---------------------------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T4S1	T4S2
	------------------------TCTGGCTCCGTGTCTTTT---------------------------------------------------------------------	18	8	5	3	6
	------------------------TCTGGCTCCGTGTCTTTTTCT------------------------------------------------------------------	21	1	1	1	1
	------------------------TCTGGCTCCGTGTCTTTTTC-------------------------------------------------------------------	20	-	-	-	2
	----------------------CTTCTGGCTCCGTGTCTT-----------------------------------------------------------------------	18	-	-	-	1
	-------------------------------------------------------------------AAGGTACAGACCCAGGGG--------------------------	18	-	-	-	1
rat	caaatagcaggggtaatttgcactTCTGGCTCCGTGTCTTTTtctctttgtctcagtgatactgtcaaaggtacagacccaggggaacacaaagcagctctgataatttga
	***************************************************************************************************************
rat	(((((..(((((....((((..(((((((.((.((..((((.......((((.....)))).....))))..)).)).)))))))....))))....)))))...))))).	0.980 -27.90

rat

chromosome:X:55242224:55242334:-1

intergenic

sblock5266_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock5266_cand 5arm	8	0	0	0	1	0	0	0	0	0
sblock5266_cand 3arm	2	0	0	0	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock5266_cand 5arm	0.000	0	0	0	0.000	0	0	0	0	0
sblock5266_cand 3arm	0.000	0	0	0	0	0	0	0	0	0

sblock5266 [novel_lenNOK_cloningOK_multiarm_DicerOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.123

no

no

0.44/0.67

18/23/0.55

0.0 0.0

2.2 1.5

9 2

0 0

2 1

0 0

26 28

4 10

5arm 3arm

1 1

nd nd

0.26 0.17

2 2

11

2

1

2

		reads	miRBase family seed
seed	---------------------------GUUUUCU----------------------------------------------------------------------------------	9	novel
seed	--------------------------------------------------------------------CAGAGGG-----------------------------------------	2	novel
		len	cloning frequencies
			T1S1	T3S1
	--------------------------AGTTTTCTGCCTTTCGCTCTGTG-------------------------------------------------------------------	23	3	-
	--------------------------AGTTTTCTGCCTTTCGCTC-----------------------------------------------------------------------	19	1	1
	--------------------------AGTTTTCTGCCTTTCGCTCTGT--------------------------------------------------------------------	22	2	-
	--------------------------AGTTTTCTGCCTTTCGCT------------------------------------------------------------------------	18	2	-
	-------------------------------------------------------------------CCAGAGGGCAGGTGGAAC-------------------------------	18	1	-
	-------------------------------------------------------------------CCAGAGGGCAGGTGGAACTGT----------------------------	21	1	-
rat	ttcccagctacaagcctgggtctcccAGTTTTCTGCCTTTCGCTCTGTGgcgtgtggatgctggttcCCAGAGGGCAGGTGGAACTGTgggcccttaggtggtctgctgcaaggag
	********************************************************************************************************************
rat	.(((((((.....(((((((..(((((((..((..(((.((.(((((((((((....)))))).....))))))).)))..)))))).)))..))))))).....))))...))).	0.580 -45.80

rat

chromosome:17:19318487:19318602:1

intergenic

sblock6558_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock6558_cand 5arm	0	0	1	3	0	1	0	0	0	0
sblock6558_cand 3arm	0	0	0	0	4	1	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock6558_cand 5arm	0	0	0.000	0.000	0	0.000	0	0	0	0
sblock6558_cand 3arm	0	0	0	0	0.000	0.000	0	0	0	0

sblock6558 [novel_lenNOK_cloningOK_multiarm_DicerOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.184

no

no

0.44/0.48

18/21/0.50

0.7 0.0 0.2

1.7 0.0 0.4

3 2 5

0 0 0

3 1 2

0 0 0

7 14 8

5 1 5

5arm 5arm 3arm

1 1 1

nd nd nd

0.40 0.32 0.26

4 2 3

10

4

1

2

Families

Member of family novel6 (seed UUGCCUG): block714821_cand, block714821_cand, block714822_cand, block714822_cand, block714823_cand, block714823_cand, block714824_cand, block714824_cand, block714825_cand, block714825_cand, sblock6558_cand

		reads	miRBase family seed
seed	--------------------------------------------UUGCCUG--------------------	4	novel
seed	---------------AGUUACU-------------------------------------------------	2	novel
seed	--------CCUCAUG--------------------------------------------------------	2	novel
seed	---------------------------------------------UGCCUGG-------------------	1	novel
seed	----------UCAUGAG------------------------------------------------------	1	novel
		len	cloning frequencies
			T2S1	T2S2	T3S1	T3S2
	-------------------------------------------ATTGCCTGGTTTAGTCTCTG--------	20	-	-	2	1
	--------------GAGTTACTAACCTCCACTC--------------------------------------	19	-	2	-	-
	--------------------------------------------TTGCCTGGTTTAGTCTCTG--------	19	-	-	1	-
	-------GCCTCATGAGTTACTAACCTC-------------------------------------------	21	-	1	-	-
	-------------------------------------------ATTGCCTGGTTTAGTCTC----------	18	-	-	1	-
	-------GCCTCATGAGTTACTAACC---------------------------------------------	19	-	-	-	1
	---------CTCATGAGTTACTAACCTCC------------------------------------------	20	1	-	-	-
rat	gttagtgGCCTCATGAGTTACTAACCTCCACTCcttatggtatATTGCCTGGTTTAGTCTCTGctacttgc
	***********************************************************************
rat	((.((((((.....(((..(((((((..((..((....)).....))...)))).)))))).)))))).))	0.980 -14.20

rat

chromosome:2:191145524:191145594:1

intergenic

sblock8470_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock8470_cand 5arm	0	0	0	0	0	0	0	0	2	0
sblock8470_cand 3arm	1	1	0	1	0	0	1	0	35	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock8470_cand 5arm	0	0	0	0	0	0	0	0	0.000	0
sblock8470_cand 3arm	0.000	0.000	0	0.000	0	0	0.000	0	0.002	0

sblock8470 [novel_lenNOK_cloningOK_multiarm_DicerOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.015

no

no

0.35/0.64

19/25/0.37

1.0 0.7 0.4

0.5 0.5 0.0

2 20 10

0 0 0

1 4 2

0 0 0

16 22 0

17 14 38

5arm 3arm 3arm

1 1 1

nd nd nd

0.41 0.30 0.61

4 3 10

41

5

3

1

Families

Member of family novel35 (seed CUUUCCU): block2017346_cand, block2017380_cand, sblock8470_cand

		reads	miRBase family seed
seed	-----------------------------------------------------------------------------UUCCUAA----------------------------------	10	novel
seed	------------------------------------------------------------------------------UCCUAAA---------------------------------	8	novel
seed	----------------------------------------------------------------------------------------------------CCCUGUA-----------	6	novel
seed	---------------------------------------------------------------------------CUUUCCU------------------------------------	5	novel
seed	---------------------------------------------------------------------------------------------------UCCCUGU------------	5	novel
seed	----------------------------------------------------------------------------------AAAGUCU-----------------------------	4	novel
seed	-----------------CUCACGC----------------------------------------------------------------------------------------------	1	novel
seed	-------------------CACGCUG--------------------------------------------------------------------------------------------	1	novel
seed	--------------------------------------------------------------------------------CUAAAGU-------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T4S1	T5S1
	----------------------------------------------------------------------------TTTCCTAAAGTCTTGATGG-----------------------	19	-	-	-	-	10
	-----------------------------------------------------------------------------TTCCTAAAGTCTTGATGGG----------------------	19	-	1	1	-	6
	---------------------------------------------------------------------------------------------------TCCCTGTATCTGGCAATCT	19	-	-	-	-	6
	--------------------------------------------------------------------------TCTTTCCTAAAGTCTTGATG------------------------	20	-	-	-	1	4
	--------------------------------------------------------------------------------------------------TTCCCTGTATCTGGCAATCT	20	-	-	-	-	4
	---------------------------------------------------------------------------------TAAAGTCTTGATGGGCCTTC-----------------	20	-	-	-	-	2
	------------------TCACGCTGCCTGGAAAGCACT-------------------------------------------------------------------------------	21	-	-	-	-	1
	--------------------------------------------------------------------------------------------------TTCCCTGTATCTGGCAATC-	19	1	-	-	-	-
	---------------------------------------------------------------------------------TAAAGTCTTGATGGGCCTTCCCTGT------------	25	-	-	-	-	1
	----------------CCTCACGCTGCCTGGAAAGCAC--------------------------------------------------------------------------------	22	-	-	-	-	1
	-------------------------------------------------------------------------------CCTAAAGTCTTGATGGGCCT-------------------	20	-	-	-	-	1
	---------------------------------------------------------------------------------TAAAGTCTTGATGGGCCTTCCC---------------	22	-	-	-	-	1
rat	atacttgggttcaggtcctcacgctgcctggaaagcactttatggactgacagtctctcaggtctgcacttcatTCTTTCCTAAAGTCTTGATGGGccTTCCCTGTATCTGGCAATCT
	**********************************************************************************************************************
rat	((((..(((...(((((((((.(((....((((((......(((((.((.(((.((....)).))))).))))).))))))...)))..))).)))))).))).))))..........	0.980 -34.20

rat

chromosome:4:116143631:116143748:-1

intergenic

sblock9284_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock9284_cand 5arm	0	0	0	0	0	0	2	0	0	0
sblock9284_cand 3arm	0	0	0	0	0	0	5	8	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock9284_cand 5arm	0	0	0	0	0	0	0.000	0	0	0
sblock9284_cand 3arm	0	0	0	0	0	0	0.000	0.000	0	0

sblock9284 [novel_lenNOK_cloningOK_multiarm_DicerOK]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.024

no

no

0.53/0.59

18/22/0.67

0.0 0.0 0.0

0.5 1.0 0.0

2 11 2

0 0 0

1 2 1

0 0 0

35 22 4

1 2 23

5arm 3arm 3arm

1 1 1

nd nd nd

0.23 0.17 0.16

3 3 1

15

2

1

3

Families

Member of family novel123 (seed CUGAGGA): block1766518_cand, sblock9284_cand

		reads	miRBase family seed
seed	---------------------------------------------------------------------------CUGAGGA------------------------------------	11	novel
seed	------------------------------------------------------------------------------------------------AGAGCAG---------------	2	novel
seed	------------------------------------GUGCCUG---------------------------------------------------------------------------	2	novel
		len	cloning frequencies
			T4S1	T4S2
	--------------------------------------------------------------------------ACTGAGGACAGCTGACACCAG-----------------------	21	1	3
	--------------------------------------------------------------------------ACTGAGGACAGCTGACACCA------------------------	20	1	2
	--------------------------------------------------------------------------ACTGAGGACAGCTGACACC-------------------------	19	2	-
	-----------------------------------------------------------------------------------------------GAGAGCAGCTGTCATGTCA----	19	-	2
	-----------------------------------GGTGCCTGCTGTCTTTCAGGTT-------------------------------------------------------------	22	1	-
	-----------------------------------GGTGCCTGCTGTCTTTCAGGT--------------------------------------------------------------	21	1	-
	--------------------------------------------------------------------------ACTGAGGACAGCTGACAC--------------------------	18	-	1
	--------------------------------------------------------------------------ACTGAGGACAGCTGACACCAGG----------------------	22	1	-
rat	ccctgactctgtgagggcccctttggcttcctcccGGTGCCTGCTGTCTTTCAGGTTgttaaggatgaatgtcaACTGAGGACAGCTGACACCAGGAGAGCAGCTGTCATGTCAtggg
	**********************************************************************************************************************
rat	(((((((...((((.(((.......(((..((((.((((...(((((((((..(((((..............))))))))))))))...)))).))))))).))).)))))))).)))	0.910 -45.74

rat

chromosome:5:26270606:26270723:-1

intergenic

novel_multiarm_DicerOK_mirtron (5 loci)

sblock11107_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock11107_cand 5arm	0	0	0	0	0	0	0	0	1	0
sblock11107_cand 3arm	1	2	0	0	0	1	0	0	1	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock11107_cand 5arm	0	0	0	0	0	0	0	0	0.000	0
sblock11107_cand 3arm	0.000	0.000	0	0	0	0.000	0	0	0.000	0

sblock11107 [novel_multiarm_DicerOK_mirtron]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.203

no

no

0.56/0.64

18/23/0.83

0.0 0.6

0.0 1.2

1 5

0 0

1 4

0 0

32 24

2 6

5arm 3arm

1 1

36 0

0.22 0.17

2 3

6

4

2

1

		reads	miRBase family seed
seed	---------------------------------------------------------------------CUGUCCG---------------------------------------	3	novel
seed	----------------------------------------------------------------------UGUCCGU--------------------------------------	2	novel
seed	---------------------------------AGGGUGG---------------------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T3S2	T5S1
	---------------------------------------------------------------------CTGTCCGTCCACCTCTTCCCAG------------------------	22	-	2	-	-
	--------------------------------------------------------------------TCTGTCCGTCCACCTCTTCCCA-------------------------	22	-	-	1	-
	--------------------------------------------------------------------TCTGTCCGTCCACCTCTT-----------------------------	18	1	-	-	-
	--------------------------------------------------------------------TCTGTCCGTCCACCTCTTCCCAG------------------------	23	-	-	-	1
	--------------------------------CAGGGTGGTGGTGGCCACAGAAA------------------------------------------------------------	23	-	-	-	1
rat	ggcacatctggatcccctctgagcctgactccCAGGGTGGTGGTGGCCACAGAAAcaaggcctgtaacTCTGTCCGTCCACCTCTTCCCAGgcgcggtatccacgacatgttccc
	*******************************************************************************************************************
	-----------------------------------------------------------------------------------------------------------------------------	ENSRNOT00000061995 ENSRNOG00000040281
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000047462 ENSRNOG00000030597 Ankrd52_predicted
rat	((.((((((((((.....(((.(((((.......(((.((((((((..(((((.(((.....)))...)))))))).)))))...)))))))).))).))))).))..))).)).	0.780 -36.71

rat

chromosome:7:1674163:1674277:1

Opposite_strand|Intronic_coding|ENSRNOT00000061995|ENSRNOG00000040281 ## Opposite_strand|Boundary_non-coding|ENSRNOT00000061993|ENSRNOG00000040281 ## Same_strand|Intronic_coding|ENSRNOT00000047462|ENSRNOG00000030597 ## ENSRNOG00000030597|protein_coding|Ankrd52_predicted| ## ENSRNOG00000040281|protein_coding||

sblock2417_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock2417_cand 5arm	1	0	0	0	0	0	0	0	0	0
sblock2417_cand 3arm	2	2	0	0	0	0	1	0	1	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock2417_cand 5arm	0.000	0	0	0	0	0	0	0	0	0
sblock2417_cand 3arm	0.000	0.000	0	0	0	0	0.000	0	0.000	0

sblock2417 [novel_multiarm_DicerOK_mirtron]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.053

no

no

0.59/0.74

19/22/0.86

0.0 0.0

0.0 0.5

1 6

0 0

1 4

0 0

28 26

4 11

5arm 3arm

1 1

40 0

0.23 0.23

3 3

7

4

2

2

		reads	miRBase family seed
seed	----------------------------------------------------------------------CGGCCCC----------------------------------------	6	novel
seed	-----------------------------GCAGGGC---------------------------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T4S1	T5S1
	---------------------------------------------------------------------TCGGCCCCTTCCTGCCCTCAG---------------------------	21	2	1	-	1
	---------------------------------------------------------------------TCGGCCCCTTCCTGCCCTC-----------------------------	19	-	1	-	-
	---------------------------------------------------------------------TCGGCCCCTTCCTGCCCTCAGA--------------------------	22	-	-	1	-
	----------------------------TGCAGGGCACTTGGGGGAGAAA-------------------------------------------------------------------	22	1	-	-	-
rat	ctgcagctctgggcagagacgggcatgcTGCAGGGCACTTGGGGGAGAAAgcctgtgcaggccttaaccTCGGCCCCTTCCTGCCCTCAGAtgccggcgacatgattgagatgcagg
	*********************************************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000056404 ENSRNOG00000027194 NP_001100490.1
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000034298 ENSRNOG00000027194 NP_001100490.1
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000047143 ENSRNOG00000027194 NP_001100490.1
rat	(((((.(((....((....(((((((.(((.((((((...(((((.((((((((....)))).))....))..)))))...))))))))))))))..))...))...))).))))).	0.840 -48.70

rat

chromosome:10:62176053:62176169:-1

Same_strand|Intronic_coding|ENSRNOT00000056404|ENSRNOG00000027194 ## Same_strand|Boundary_coding|ENSRNOT00000056404|ENSRNOG00000027194 ## ENSRNOG00000027194|protein_coding|NP_001100490.1|RUN and TBC1 domain containing 1 [Source:RefSeq_peptide;Acc:NP_001100490]

sblock2425_cand

Cloning frequencies

absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock2425_cand 5arm	0	0	1	0	0	0	0	0	0	0
sblock2425_cand 3arm	1.500	1	1	0	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
sblock2425_cand 5arm	0	0	0.000	0	0	0	0	0	0	0
sblock2425_cand 3arm	0.000	0.000	0.000	0	0	0	0	0	0	0

sblock2425 [novel_multiarm_DicerOK_mirtron]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.187

no

no

0.48/0.62

20/22/1.00

0.0 0.0

0.0 1.2

1 4

0 0

1 3

0 0

26 27

5 8

5arm 3arm

1 2

41 0

0.29 0.33

3 3

5

3

1

1

		reads	miRBase family seed
seed	-------------------------------------------------------------------GUUCCUG-----------------------------------------	4	novel
seed	---------------------------GGGUCUU---------------------------------------------------------------------------------	1	miR-193*
		len	cloning frequencies
			T1S1	T1S2	T2S1
	------------------------------------------------------------------TGTTCCTGTGCCATTTCCTCAG---------------------------	22	-	1	1
	------------------------------------------------------------------TGTTCCTGTGCCATTTCCTCA----------------------------	21	1	-	-
	--------------------------TGGGTCTTGGCGTTGGCACAC--------------------------------------------------------------------	21	-	-	1
	------------------------------------------------------------------TGTTCCTGTGCCATTTCCTC-----------------------------	20	1	-	-
rat	tggttctccgtaaaactgagaatgacTGGGTCTTGGCGTTGGCACACctgttcacagcaaccttggTGTTCCTGTGCCATTTCCTCAGgctgctctttgggattgctcagcccca
	*******************************************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000015207 ENSRNOG00000011366 Pigs
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000056222 ENSRNOG00000011366 Pigs
rat	.((..((..((((..(((((.....(((((...(((((..((.(((((.(((......)))...))))).))..)))))...)).)))....)))...))..))))..))..)).	0.450 -34.60

rat

chromosome:10:64293651:64293765:-1

Same_strand|Intronic_coding|ENSRNOT00000056222|ENSRNOG00000011366 ## ENSRNOG00000011366|protein_coding|Pigs|GPI transamidase component PIG-S (Phosphatidylinositol-glycan biosynthesis class S protein). [Source:UniProtKB/Swiss-Prot;Acc:Q5XI31]

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candidate novel miRNAs:

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