logoSmall RNA from different tissues of two rat strains.


miR classification

Alignments

other hairpins

novel_lenNOK_gcHIGH_multiarm_DicerNOK (14 loci)

sblock4823 [novel_lenNOK_gcHIGH_multiarm_DicerNOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.931nono0.89/0.9518/19/0.000.0
0.0
0.0
0.0
0.0
0.0
1
1
2
0
0
0
1
1
2
0
0
0
29
23
14
8
10
20
5arm
3arm
3arm
1
1
1
nd
nd
nd
0.33
0.11
0.39
2
1
5
4338
  readsmiRBase family seed
seed     ------------------------------------------------------------------------------------CCAGGCG------------------------     2novel
seed     ------------------------------GCGCGCC------------------------------------------------------------------------------     1novel
seed     --------------------------------------------------------------------------CGGCGGC----------------------------------     1novel
  lencloning frequencies
   T1S1T4S1T5S2
      -----------------------------------------------------------------------------------GCCAGGCGCGGGGACCGG--------------     1811-
      -------------------------------------------------------------------------CCGGCGGCGCGCCAGGCGC-----------------------     19--1
      -----------------------------CGCGCGCCGGCTCGGGGC--------------------------------------------------------------------     18-1-
rat     cgggcggcggggcgcgcgggagcacctggCGCGCGCCGGCTCGGGGCccgcgcgccctcctttgtgcgcggacCCGGCGGCGCGCCAGGCGCGGGGACCGGcggcgggagttcgg      
      *******************************************************************************************************************      
 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ENSRNOT00000047215 ENSRNOG00000012934 Arhgef7
rat     (((((......((.(((.((.((.((((((..(((((.((.((((..((((((((........)))))))).))))))))))))))))).)).....)).)))))....))))).     0.950 -67.90

ratchromosome:16:82635146:82635260:-1Same_strand|Boundary_non-coding|ENSRNOT00000017947|ENSRNOG00000012934 ## Same_strand|Intronic_coding|ENSRNOT00000047215|ENSRNOG00000012934 ## ENSRNOG00000012934|protein_coding|Arhgef7|Rho guanine nucleotide exchange factor 7 (PAK-interacting exchange factor beta) (Beta-Pix). [Source:UniProtKB/Swiss-Prot;Acc:O55043] ## {Repeats: dust 82635169 82635188 0 class=dust,GC_rich 17 36 -1 class=Low_complexity,GC_rich 11 29 -1 class=Low_complexity,dust 82635160 82635178 0 class=dust,dust 82635214 82635232 0 class=dust,GC_rich 1 36 -1 class=Low_complexity,GC_rich 8 26 -1 class=Low_complexity,GC_rich 1 76 -1 class=Low_complexity} ## {SimpF: oe = 0.96 0 CpG, -1 Eponine}


sblock5675 [novel_lenNOK_gcHIGH_multiarm_DicerNOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.382nono0.89/0.9418/19/0.000.0
0.0
0.0
0.0
1
1
0
0
1
1
0
0
34
31
1
1
5arm
3arm
1
1
nd
nd
0.11
0.17
1
1
220-2
  readsmiRBase family seed
seed-----------------------------------CCGAGCG----------------------------------------------------------------------------1novel
seed----------------------------------------------------------------------GGGGCCG-----------------------------------------1novel
  lencloning frequencies
   T3S1T4S2
 ---------------------------------------------------------------------CGGGGCCGGCCGCGCTCG-------------------------------18-1
 ----------------------------------ACCGAGCGCCGCGGCCCCG-----------------------------------------------------------------191-
ratggcgggctgagcggccccgcctcccgcgtgctgcACCGAGCGCCGCGGCCCCGccgcgcctttaaaccgCGGGGCCGGCCGCGCTCGcgggcacttggcggctggcggcgagcgtgcc 
 ********************************************************************************************************************** 
rat((((.(((..((.(((..(((..(((.(((((....(((((((.((((((((((..............)))))))).)).)))))))..))))).))).))).))).)).))).))))0.870 -70.94

ratchromosome:18:53673092:53673209:1intergenic ## {Repeats: GC_rich 1 21 1 class=Low_complexity,GC_rich 1 14 1 class=Low_complexity} ## {SimpF: 1 Eponine,oe = 0.86 1 CpG, -1 Eponine}


sblock5742 [novel_lenNOK_gcHIGH_multiarm_DicerNOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.416nono0.50/0.9418/18/0.000.0
0.0
0.0
0.0
1
1
0
0
1
1
0
0
1
11
32
17
5arm
3arm
1
1
nd
nd
0.50
0.22
7
2
22-12-10
  readsmiRBase family seed
seed     --------------------------------------------------------------------------GCCGGGC---------------------     1novel
seed     --CUCCAAC---------------------------------------------------------------------------------------------     1novel
  lencloning frequencies
   T1S1T3S1
      -GCTCCAACAACCTTCAGT-----------------------------------------------------------------------------------     181-
      -------------------------------------------------------------------------GGCCGGGCGGGGAGGGGG-----------     18-1
rat     gGCTCCAACAACCTTCAGTcctcgcactatgatccttttgttcttttatagcaagactgtagaaataggctggGGCCGGGCGGGGAGGGGGcaggcaaggcc      
      ******************************************************************************************************      
 ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ENSRNOT00000019301 ENSRNOG00000014293 NP_001102364.1
rat     ((((.......(((((..(((((((....((..(((...(((.((((((((.....))))))))...))).)))..)).)))))))))))).......))))     0.840 -32.04

ratchromosome:19:19827185:19827286:-1Same_strand|Intronic_coding|ENSRNOT00000019301|ENSRNOG00000014293 ## ENSRNOG00000014293|protein_coding|NP_001102364.1|naked cuticle 1 homolog [Source:RefSeq_peptide;Acc:NP_001102364]


sblock5748 [novel_lenNOK_gcHIGH_multiarm_DicerNOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.089nono0.69/0.9418/26/0.000.0
0.0
0.0
0.0
1
1
0
0
1
1
0
0
14
8
14
10
5arm
3arm
1
1
nd
nd
0.17
0.23
1
2
22-44
  readsmiRBase family seed
seed     ---------------CCCGGCC----------------------------------------------------------------------------     1novel
seed     -----------------------------------------------------------------AGGCGCC--------------------------     1novel
  lencloning frequencies
   T3S2T4S2
      --------------CCCCGGCCCGTCGGGGCG------------------------------------------------------------------     18-1
      ----------------------------------------------------------------CAGGCGCCTGGGCAGGCTGGTACAGA--------     261-
rat     gggtcggactttgcCCCCGGCCCGTCGGGGCGcggagggggcgggataatgctttcaggcatccCAGGCGCCTGGGCAGGCTGGTACAGAgctgcctc      
      **************************************************************************************************      
 ------------------------------------------------------------------------------------------------------------ENSRNOT00000005122 ENSRNOG00000003747 Asna1
rat     (((.(((.(((((...((((((.(((.((((((...((((((..((........))..)).))))..)))))).))).))))))..))))))))))).     0.910 -49.60

ratchromosome:19:24829720:24829817:-1Opposite_strand|Intronic_non-coding|ENSRNOT00000005122|ENSRNOG00000003747 ## ENSRNOG00000003747|protein_coding|Asna1|Asna1 protein (Fragment). [Source:UniProtKB/TrEMBL;Acc:Q4G022]


sblock591 [novel_lenNOK_gcHIGH_multiarm_DicerNOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.521nono0.71/1.0018/22/0.500.0
0.5
1.0
0.0
0.0
0.7
1
2
3
0
0
0
1
1
2
0
0
0
25
26
1
16
5
25
5arm
3arm
3arm
9
9
9
nd
nd
nd
0.28
0.16
0.32
2
2
4
64-20
  readsmiRBase family seed
seed     ------------------------------------------------------------------------------------------UGUGGGG---------------     1novel
seed     -----------------------------------------------------------------------------------------CUGUGGG----------------     1novel
seed     --------------------------GGGCGGG-------------------------------------------------------------------------------     1novel
seed     ----------------------------------------------------------------------------------------CCUGUGG-----------------     1novel
seed     ---------------------------------------------------------------------CCGUGGC------------------------------------     1novel
seed     --------------------------------------------------------------------CCCGUGG-------------------------------------     1novel
  lencloning frequencies
   T1S1T1S2T3S2T5S2
      ---------------------------------------------------------------------------------------GCCTGTGGGGACGGAGGAGTGC---     22---1
      ----------------------------------------------------------------------------------------CCTGTGGGGACGGAGGAGTGC---     21---1
      -------------------------------------------------------------------CCCCGTGGCGCCTACCCTC--------------------------     191---
      -----------------------------------------------------------------------------------------CTGTGGGGACGGAGGAGTGCGC-     22--1-
      --------------------------------------------------------------------CCCGTGGCGCCTACCCTC--------------------------     181---
      -------------------------GGGGCGGGGCGGCGGCGG---------------------------------------------------------------------     18-1--
rat     cggcgcgccggcttcctgcttggcaGGGGCGGGGCGGCGGCGGcgattgtctggcggcctggggcggCCCCGTGGCGCCTACCCTCtGCCTGTGGGGACGGAGGAGTGCGCc      
      ****************************************************************************************************************      
 .....................................................................................................................>>>>>ENSRNOT00000018194 ENSRNOG00000013381 Tsg101
rat     .(((((((...(((((..(((((((((((..((((.((.((((.........(((.(((...))).))))))).))))))..))))))))....)))..))))).)))))))     0.490 -61.80

ratchromosome:1:97475101:97475212:-1intergenic ## {Repeats: dust 97475144 97475146 0 class=dust,dust 97475170 97475188 0 class=dust}


sblock7589 [novel_lenNOK_gcHIGH_multiarm_DicerNOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.450nono0.83/1.0018/19/0.000.0
0.0
0.0
0.0
1
1
0
0
1
1
0
0
28
20
7
17
5arm
3arm
1
1
nd
nd
0.28
0.11
4
1
21711
  readsmiRBase family seed
seed     ----------------------------------------------------------------------------------CGGCGCG-------------------------------     1novel
seed     -----------------------------GCGCAGG------------------------------------------------------------------------------------     1novel
  lencloning frequencies
   T1S1
      ----------------------------CGCGCAGGGGCCGGAGGA--------------------------------------------------------------------------     181
      ---------------------------------------------------------------------------------GCGGCGCGGGGCGGGCGGG--------------------     191
rat     tcgcgcctcgcgggcgcacgcgccgcctCGCGCAGGGGCCGGAGGAggccgggcctgtcagaccccgcggccggcctccgaGCGGCGCGGGGCGGGCGGGtgcagcggcttgggctcggc      
      ************************************************************************************************************************      
 ..........................................................................................>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>ENSRNOT00000000184 ENSRNOG00000000170 Slc30a4
rat     (((.(((((((..((((.(((.(((((((((((....((((((((.(((((((...........)).)))))..)))))).)).)))))))))))))).)))).)))....)))).))).     1.000 -76.90

ratchromosome:3:109665716:109665835:-1Same_strand|Boundary_non-coding|ENSRNOT00000000184|ENSRNOG00000000170 ## intergenic ## ENSRNOG00000000170|protein_coding|Slc30a4|Zinc transporter 4 (ZnT-4) (Solute carrier family 30 member 4) (Dri 27 protein). [Source:UniProtKB/Swiss-Prot;Acc:O55174] ## {Repeats: GC_rich 1 20 -1 class=Low_complexity,GC_rich 1 185 -1 class=Low_complexity,GC_rich 55 73 -1 class=Low_complexity} ## {SimpF: 1 Eponine, -1 Eponine}


sblock9666 [novel_lenNOK_gcHIGH_multiarm_DicerNOK]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.416nono0.90/0.9518/22/0.501.0
0.0
1.0
0.0
3
1
0
0
2
1
0
0
11
24
20
13
5arm
3arm
1
1
nd
nd
0.23
0.17
2
1
42-7-10
  readsmiRBase family seed
seed------------GCGCGGG------------------------------------------------------------------------------------------------1novel
seed--------------------------------------------------------------------------CCCCCCG----------------------------------1novel
seed--------------GCGGGCG----------------------------------------------------------------------------------------------1novel
seed-------------CGCGGGC-----------------------------------------------------------------------------------------------1novel
  lencloning frequencies
   T1S2T3S2
 -------------------------------------------------------------------------GCCCCCCGCTCCGGCCCG------------------------18-1
 -----------CGCGCGGGCGCGGGGCGCTGAG----------------------------------------------------------------------------------221-
 ------------GCGCGGGCGCGGGGCGCTG------------------------------------------------------------------------------------191-
 -------------CGCGGGCGCGGGGCGCTGAGC---------------------------------------------------------------------------------21-1
ratgcggcggcgggCGCGCGGGCGCGGGGCGCTGAGCgcggggctcggggacggcggcgccccccgcctcgcccccGCCCCCCGCTCCGGCCCGaccacgccgcgctcgccgggagcg 
 ******************************************************************************************************************* 
rat((..(((((((((((((((..((((.((..(((((.(((((..((((..(((((......)))))...)))).))))).))))))).)))).)).))).))))))))))...)).1.000 -82.10

ratchromosome:5:22530355:22530469:1intergenic ## {Repeats: dust 22530346 22530353 0 class=dust,dust 22530392 22530415 0 class=dust,GC_rich 82 100 1 class=Low_complexity,GC_rich 1 141 1 class=Low_complexity,GC_rich 20 43 1 class=Low_complexity,trf 14 37 0 class=trf} ## {SimpF: 1 Eponine,oe = 0.96 0 CpG}


novel_lenNOK_gcHIGH_shortStem_nonpairedHIGH_bulgeHIGH (14 loci)

block69591 [novel_lenNOK_gcHIGH_shortStem_nonpairedHIGH_bulgeHIGH]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.042nono0.94/0.9418/18/0.000.0
0.0
1
0
1
0
4
31
5arm
1
nd
0.50
4
11nana
  readsmiRBase family seed
seed-----GGCGCCC------------------------------------------------------------------------------------------------1novel
  lencloning frequencies
   T1S2
 ----CGGCGCCCCTCCCCCGCC--------------------------------------------------------------------------------------181
rataaccCGGCGCCCCTCCCCCGCCccccagcaggaggcctgaggccaagctggttaaaaaaaaaagtgcacaggccggctgcagcggctaaggggattagaagagggttg 
 ************************************************************************************************************ 
rat(((((....(((((...((((....((((....((((((..((...(((..((.....))..))))).)))))).))))..))))...))))).........))))).0.980 -41.42

ratchromosome:10:37380407:37380514:1intergenic ## {Repeats: dust 37380420 37380432 0 class=dust}


block103836 [novel_lenNOK_gcHIGH_shortStem_nonpairedHIGH_bulgeHIGH]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.705nono0.95/0.9519/19/0.000.0
0.0
1
0
1
0
3
21
5arm
1
nd
0.37
4
11nana
  readsmiRBase family seed
seed     ----CCGCCGC-------------------------------------------------------------------------------------------     1novel
  lencloning frequencies
   T3S2
      ---GCCGCCGCCGCCGCCGACC--------------------------------------------------------------------------------     191
rat     cccGCCGCCGCCGCCGCCGACCcgggccccgcgcgcccaacgctcttctcacacgcgccgggcgccgcggaggaaccagcgcggggccaccgggcctgcggg      
      ******************************************************************************************************      
 ----------------------------------------------------------------------------------------------------------------ENSRNOT00000055031 ENSRNOG00000003670 LOC690244
rat     (((((.((((((.(((.((....((.((((((((((((..(((...........)))..)))))).)))).))..))..)))))))).....)))..)))))     0.420 -51.90

ratchromosome:10:105620590:105620691:1Opposite_strand|Intronic_coding|ENSRNOT00000055031|ENSRNOG00000003670 ## Opposite_strand|Boundary_non-coding|ENSRNOT00000004867|ENSRNOG00000003670 ## ENSRNOG00000003670|protein_coding|LOC690244| ## {Repeats: GC_rich 1 38 1 class=Low_complexity,GC_rich 3 22 1 class=Low_complexity,dust 105620570 105620589 0 class=dust} ## {SimpF: 1 Eponine, -1 Eponine}


block187231 [novel_lenNOK_gcHIGH_shortStem_nonpairedHIGH_bulgeHIGH]

Hairpin parameters
miRNArandfoldRNArepGC min/maxlen min/max/mir_range5'var3'varreadsreads editedlibslibs editedbase distanceloop distancepositionlocationsexon distanceunpairedmax bulgetotal readstotal libsdicerdrosha
no0.058nono1.00/1.0018/18/0.000.0
0.0
1
0
1
0
4
25
5arm
1
nd
0.44
4
11nana
  readsmiRBase family seed
seed     -----CCCGCCC----------------------------------------------------------------------------------------     1novel
  lencloning frequencies
   T5S1
      ----CCCCGCCCCCGGGGCCGG------------------------------------------------------------------------------     181
rat     ctgtCCCCGCCCCCGGGGCCGGtttcttctgatcattggcgggctccagcttcgggtcccgccccctgtcccgctccccggctcccattgtctccgcaga      
      ****************************************************************************************************      
 ...................................>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>ENSRNOT00000001849 ENSRNOG00000001367 Gpc2
rat     ((((....((....((((((((........(((....((((((..((......))..))))))....))).......))))))))....))....)))).     0.810 -36.56

ratchromosome:12:17864618:17864717:-1intergenic


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