miR-Intess™

other hairpins

novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH (11 loci)

block1374318 [novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.923

no

no

0.37/0.44

18/20/0.74

0.0

0.0

58

0

9

0

10

-5

5arm_loop

1

nd

0.55

6

106

9

na

na

		reads	miRBase family seed
seed	-----------AGAAAGU----------------------------------------------------	100	novel
seed	-------------AAAGUAU--------------------------------------------------	5	novel
seed	------------GAAAGUA---------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	----------AAGAAAGTATGCAGGAACTC----------------------------------------	20	3	-	6	4	-	5	2	31	27
	----------AAGAAAGTATGCAGGAAC------------------------------------------	18	2	2	1	-	2	-	-	4	2
	----------AAGAAAGTATGCAGGAACT-----------------------------------------	19	1	1	1	1	-	1	-	2	2
	------------GAAAGTATGCAGGAACTC----------------------------------------	18	-	3	-	1	-	1	-	-	-
	-----------AGAAAGTATGCAGGAACTC----------------------------------------	19	-	-	-	-	-	-	-	1	-
rat	ctgtccctttAAGAAAGTATGCAGGAACTCagcaacatcatctgccttctaatttgcctgggtgttacag
	**********************************************************************
rat	(((((((..((((..((...(((((...............)))))...))..))))...)))....))))	0.490 -9.06

rat

chromosome:3:57017407:57017476:-1

intergenic

block1413520 [novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.400

no

no

0.40/0.45

18/25/0.95

0.0

0.0

45

0

5

0

8

-3

5arm_loop

1

nd

0.40

4

60

5

na

na

		reads	miRBase family seed
seed	---------AGACAUC----------------------------------------------	58	novel
seed	----------GACAUCC---------------------------------------------	2	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T4S2	T5S1
	--------GAGACATCCACAGTTGTACT----------------------------------	20	2	2	1	3	45
	---------AGACATCCACAGTTGTACTA---------------------------------	20	-	-	-	-	2
	--------GAGACATCCACAGTTGTA------------------------------------	18	-	-	-	1	1
	--------GAGACATCCACAGTTGTACTAT--------------------------------	22	1	-	-	-	1
	--------GAGACATCCACAGTTGTACTATGCT-----------------------------	25	-	-	-	-	1
rat	tacatcagGAGACATCCACAGTTGTACTatgctttgatgattgttgagctaaaatgttgtga
	**************************************************************
rat	((((.((..((...((.((((((((...........)))))))).)).))....)).)))).	0.760 -9.40

rat

chromosome:3:141076517:141076578:-1

intergenic

block2311834 [novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.334

no

no

0.46/0.55

19/24/0.96

0.4

0.0

37

0

4

0

30

-4

3arm_loop

1

nd

0.52

4

50

4

na

na

		reads	miRBase family seed
seed	---------------------------------------------------------------GACAGAU---------------------------------------------	28	novel
seed	--------------------------------------------------------------UGACAGA----------------------------------------------	20	novel
seed	--------------------------------------------------------GGUCCUU----------------------------------------------------	1	novel
seed	-----------------------------------------------------------CCUUGAC-------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T3S2	T4S1	T5S1
	--------------------------------------------------------------TGACAGATGGCTGAGATCAGAGA------------------------------	23	1	-	1	23
	-------------------------------------------------------------TTGACAGATGGCTGAGATCAGAGA------------------------------	24	2	1	-	14
	-------------------------------------------------------------TTGACAGATGGCTGAGATCAGAG-------------------------------	23	-	-	-	2
	--------------------------------------------------------------TGACAGATGGCTGAGATCAGAG-------------------------------	22	1	-	-	1
	-------------------------------------------------------TGGTCCTTGACAGATGGCTG----------------------------------------	20	-	-	-	1
	--------------------------------------------------------------TGACAGATGGCTGAGATCA----------------------------------	19	-	-	-	1
	-------------------------------------------------------------TTGACAGATGGCTGAGATCAG---------------------------------	21	-	-	-	1
	----------------------------------------------------------TCCTTGACAGATGGCTGAG--------------------------------------	19	-	-	-	1
rat	gaggtcttagctgagtagacaggagcaaccggtgcctgaaagatgatggcctgcatggtccTTGACAGATGGCTGAGATCAGAGAaggtagctgtgatcctgtgtcctgaggtct
	*******************************************************************************************************************
rat	.((..(((((..((....((((((.((..((((((((......((((((((....((........))...))))...))))....))))).))))).)))))).)))))))..))	0.780 -32.90

rat

chromosome:8:57733296:57733410:-1

intergenic

block1060732 [novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.056

no

no

0.40/0.68

19/26/0.81

0.3 0.0

0.0 0.0

29 4

0 0

4 2

0 0

28 9

-1 19

3arm 3arm

1 1

nd nd

0.32 0.38

7 3

63

5

na

na

		reads	miRBase family seed
seed	----------------------------------------------GGUCAGA------------------------------------------	21	miR-540
seed	-----------------------------------------------GUCAGAG-----------------------------------------	12	novel
seed	--------------------------------------------AAGGUCA--------------------------------------------	8	novel
seed	-------------------------------------------------CAGAGAA---------------------------------------	5	novel
seed	------------------------------------------------------------------GCAAACC----------------------	5	novel
seed	------------------------------------------------------AACUCUU----------------------------------	3	novel
seed	--------------------------------------------------------CUCUUCU--------------------------------	2	novel
seed	-------------------------------------------------------ACUCUUC---------------------------------	2	novel
seed	----------------------------------------------------------------GUGCAAA------------------------	2	miR-19a/19b
seed	--------------------------------------------------------------UUGUGCA--------------------------	1	novel
seed	---------------------------------------------AGGUCAG-------------------------------------------	1	novel
seed	--------------------------------------------------AGAGAAC--------------------------------------	1	novel
		len	cloning frequencies
			T1S2	T3S1	T4S1	T4S2	T5S1
	---------------------------------------------AGGTCAGAGAACTCTTCTTGTG----------------------------	22	-	-	-	1	20
	----------------------------------------------GGTCAGAGAACTCTTCTTGTG----------------------------	21	1	-	-	1	9
	------------------------------------------------TCAGAGAACTCTTCTTGTG----------------------------	19	-	-	-	-	5
	-------------------------------------------TAAGGTCAGAGAACTCTTCTTGTG----------------------------	24	-	-	-	-	5
	-----------------------------------------------------------------TGCAAACCTCGGGGTGGGGTG---------	21	-	-	-	-	4
	-------------------------------------------------------ACTCTTCTTGTGCAAACCTC--------------------	20	-	-	-	-	2
	-----------------------------------------------------GAACTCTTCTTGTGCAAACCTC--------------------	22	-	-	-	-	2
	------------------------------------------------------AACTCTTCTTGTGCAAACCTC--------------------	21	-	-	-	-	2
	--------------------------------------------AAGGTCAGAGAACTCTTCT--------------------------------	19	-	1	-	-	-
	-------------------------------------------TAAGGTCAGAGAACTCTTCT--------------------------------	20	-	-	-	-	1
	-------------------------------------------------CAGAGAACTCTTCTTGTGCAAACCTC--------------------	26	-	-	-	-	1
	-------------------------------------------------------------CTTGTGCAAACCTCGGGGTGGG------------	22	-	-	1	-	-
	---------------------------------------------------------------TGTGCAAACCTCGGGGTGG-------------	19	-	-	1	-	-
	----------------------------------------------GGTCAGAGAACTCTTCTTG------------------------------	19	-	-	-	-	1
	---------------------------------------------------------------TGTGCAAACCTCGGGGTGGGGT----------	22	-	-	1	-	-
	-------------------------------------------TAAGGTCAGAGAACTCTTC---------------------------------	19	-	-	-	-	1
	-----------------------------------------------------GAACTCTTCTTGTGCAAAC-----------------------	19	1	-	-	-	-
	-----------------------------------------------------------------TGCAAACCTCGGGGTGGGG-----------	19	-	-	-	-	1
	-------------------------------------------TAAGGTCAGAGAACTCTTCTTG------------------------------	22	-	-	-	-	1
rat	ctcaatttcccatacgtctttgcgagttttctgatcaggggagtaAGGTCAGAGAACTCTTCTTGTGcaaacctcggggtggggtgccttggagg
	***********************************************************************************************
rat	(((....((((((.((..((((((((((((((((((..........))))))))))))).......)))))...))..)))))).......))).	0.970 -32.41

rat

chromosome:20:5515117:5515211:-1

intergenic

block1093656 [novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.845

no

no

0.37/0.48

18/25/0.83

0.5

0.8

27

0

8

0

38

-9

3arm

1

nd

0.45

4

72

8

0

-1

		reads	miRBase family seed
seed	-----------------------------------------GGUUUCG------------------------------------------------------------	35	novel
seed	---------------------------------------CUGGUUU--------------------------------------------------------------	13	novel
seed	----------------------------------------------------GACUCAA-------------------------------------------------	7	novel
seed	--------------------------------GCUGUAU---------------------------------------------------------------------	3	novel
seed	----------------------------------------------CGUGAUG-------------------------------------------------------	3	novel
seed	-------------------------------------------------GAUGACU----------------------------------------------------	2	novel
seed	----------------------------------UGUAUCU-------------------------------------------------------------------	2	novel
seed	--------------------------------------------------------CAAGGAU---------------------------------------------	2	novel
seed	-----------------------------------GUAUCUG------------------------------------------------------------------	2	novel
seed	----------------------------------------UGGUUUC-------------------------------------------------------------	1	miR-29b
seed	-------------------------------------AUCUGGU----------------------------------------------------------------	1	novel
seed	----------------------------------------------------------AGGAUUC-------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T5S1
	----------------------------------------TGGTTTCGTGATGACTCAAG------------------------------------------------	20	2	2	-	1	-	1	1	20
	--------------------------------------TCTGGTTTCGTGATGACTC---------------------------------------------------	19	-	-	-	-	-	-	-	7
	----------------------------------------TGGTTTCGTGATGACTCAAGGAT---------------------------------------------	23	-	-	-	-	-	-	-	5
	--------------------------------------TCTGGTTTCGTGATGACTCAAGGA----------------------------------------------	24	-	-	-	-	-	-	-	4
	---------------------------------------------TCGTGATGACTCAAGGATTCTGT----------------------------------------	23	-	-	-	-	-	-	-	3
	------------------------------------------------TGATGACTCAAGGATTCTGT----------------------------------------	20	-	-	-	-	-	-	-	2
	-------------------------------TGCTGTATCTGGTTTCGTGA---------------------------------------------------------	20	-	-	-	-	-	-	-	2
	---------------------------------------------------TGACTCAAGGATTCTGTTAACC-----------------------------------	22	-	-	-	-	-	-	-	2
	----------------------------------------TGGTTTCGTGATGACTCAAGGA----------------------------------------------	22	-	-	-	-	-	-	-	2
	-------------------------------------------------------TCAAGGATTCTGTTAACCAG---------------------------------	20	-	-	-	-	-	-	-	2
	---------------------------------------------------TGACTCAAGGATTCTGTTAACCA----------------------------------	23	-	-	-	-	-	-	-	2
	---------------------------------------------------TGACTCAAGGATTCTGTTAAC------------------------------------	21	-	-	-	-	-	-	-	1
	--------------------------------------TCTGGTTTCGTGATGACT----------------------------------------------------	18	-	-	-	-	1	-	-	-
	----------------------------------------TGGTTTCGTGATGACTCAAGG-----------------------------------------------	21	-	-	-	-	-	-	-	1
	---------------------------------------CTGGTTTCGTGATGACTCAAGGA----------------------------------------------	23	-	-	-	-	-	-	-	1
	---------------------------------------------------TGACTCAAGGATTCTGTTA--------------------------------------	19	-	-	-	-	-	-	-	1
	---------------------------------------------------------AAGGATTCTGTTAACCAGC--------------------------------	19	-	-	-	-	-	-	-	1
	--------------------------------------TCTGGTTTCGTGATGACTCA--------------------------------------------------	20	-	-	1	-	-	-	-	-
	---------------------------------CTGTATCTGGTTTCGTGATGACTCA--------------------------------------------------	25	-	-	-	-	-	-	-	1
	----------------------------------TGTATCTGGTTTCGTGATGACT----------------------------------------------------	22	-	-	-	-	-	-	-	1
	---------------------------------------------------TGACTCAAGGATTCTGTTAACCAG---------------------------------	24	-	-	-	-	-	-	-	1
	-------------------------------TGCTGTATCTGGTTTCGT-----------------------------------------------------------	18	-	-	-	-	-	-	-	1
	------------------------------------TATCTGGTTTCGTGATGACTC---------------------------------------------------	21	-	-	-	-	-	-	-	1
	----------------------------------TGTATCTGGTTTCGTGATGAC-----------------------------------------------------	21	-	-	-	-	-	-	-	1
	---------------------------------CTGTATCTGGTTTCGTGATGAC-----------------------------------------------------	22	-	-	-	-	-	-	-	1
rat	ggagaaatgggaccccaaagaagagaggccctgctgtaTCTGGTTTCGTGATGACTCAAGgattctgttaaccagccaaggtccttctggtggcttcctggaagcttt
	************************************************************************************************************
rat	.(((...(((((..(((.(((((...((((..((((.....((..((.(((....)))..))..))......))))...)))))))))..)))..)))))....))).	0.970 -26.80

rat

chromosome:20:5543929:5544036:1

intergenic

block881562 [novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.442

no

no

0.32/0.38

19/24/0.87

1.0

0.6

27

0

7

0

22

-3

3arm

1

nd

0.45

4

54

7

na

na

		reads	miRBase family seed
seed	-------------------------------------------CCACACU-------------------------------------	25	novel
seed	----------------------------------------UCUCCAC----------------------------------------	19	novel
seed	---------------------------------------------ACACUUC-----------------------------------	6	novel
seed	-----------------------------------AACAUUC---------------------------------------------	2	miR-543*
seed	-----------------------------------------CUCCACA---------------------------------------	1	novel
seed	--------------------------------------------CACACUU------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T4S2	T5S1	T5S2
	------------------------------------------TCCACACTTCTTAAATGAGT-------------------------	20	3	3	1	-	1	16	-
	---------------------------------------TTCTCCACACTTCTTAAATGAGT-------------------------	23	3	1	-	-	1	6	1
	--------------------------------------------CACACTTCTTAAATGAGTACT----------------------	21	-	-	-	-	-	5	-
	---------------------------------------TTCTCCACACTTCTTAAAT-----------------------------	19	1	-	-	1	-	3	-
	----------------------------------GAACATTCTCCACACTTCTTAAA------------------------------	23	-	2	-	-	-	-	-
	---------------------------------------TTCTCCACACTTCTTAAATGA---------------------------	21	-	-	-	-	-	2	-
	-------------------------------------------CCACACTTCTTAAATGAGT-------------------------	19	-	-	-	-	-	1	-
	--------------------------------------------CACACTTCTTAAATGAGTACTTCC-------------------	24	-	-	-	-	-	1	-
	------------------------------------------TCCACACTTCTTAAATGAG--------------------------	19	-	-	-	-	-	1	-
	----------------------------------------TCTCCACACTTCTTAAATGAGT-------------------------	22	-	1	-	-	-	-	-
rat	ggtgacttgaaggcagatctcagacaaggttctggaacaTTCTCCACACTTCTTAAATGAGTACTtccactctgctgctgcataact
	***************************************************************************************
	-------------------------------------------------------------------------------------------------	ENSRNOT00000016427 ENSRNOG00000012031 St8sia2
rat	(((....((..((((((.((((...((((...((((......))))....))))...)))).........))))))....))..)))	0.740 -16.70

rat

chromosome:1:129022463:129022549:-1

Opposite_strand|Intronic_coding|ENSRNOT00000016427|ENSRNOG00000012031 ## ENSRNOG00000012031|protein_coding|St8sia2|Alpha-2,8-sialyltransferase 8B (EC 2.4.99.-) (ST8Sia II) (Sialyltransferase X) (STX). [Source:UniProtKB/Swiss-Prot;Acc:Q07977]

block1574954 [novel_cloningHIGH_loopOverlap_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.151

no

no

0.40/0.60

18/25/0.77

0.0

0.0

41

0

3

0

47

-10

5arm_loop_3arm

1

nd

0.38

5

75

3

na

na

		reads	miRBase family seed
seed	------------------------------------------------GGAAAUA--------------------------------------------------	50	novel
seed	----------------------------------GGGAAAC----------------------------------------------------------------	9	novel
seed	------------------------------GCCUGGG--------------------------------------------------------------------	5	novel
seed	--------------------------------------------GUGUGGA------------------------------------------------------	3	novel
seed	-------------------------------CCUGGGA-------------------------------------------------------------------	3	novel
seed	-------------------------------------------------GAAAUAG-------------------------------------------------	2	novel
seed	-----------------------------UGCCUGG---------------------------------------------------------------------	2	novel
seed	-------------------------------------AAACUCU-------------------------------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T5S1
	-----------------------------------------------TGGAAATAGGTGGTAAACCTG-------------------------------------	21	2	-	41
	---------------------------------TGGGAAACTCTGTGTGGAA-----------------------------------------------------	19	-	-	5
	-----------------------------------------------TGGAAATAGGTGGTAAACC---------------------------------------	19	1	-	4
	---------------------------------TGGGAAACTCTGTGTGGA------------------------------------------------------	18	-	-	3
	-----------------------------TGCCTGGGAAACTCTGTGTGGA------------------------------------------------------	22	-	-	2
	----------------------------TTGCCTGGGAAACTCTGTGTGG-------------------------------------------------------	22	-	-	2
	-------------------------------------------TGTGTGGAAATAGGTGGT--------------------------------------------	18	-	-	2
	-----------------------------TGCCTGGGAAACTCTGTGTGG-------------------------------------------------------	21	-	-	2
	-----------------------------------------------TGGAAATAGGTGGTAAACCT--------------------------------------	20	-	-	2
	------------------------------GCCTGGGAAACTCTGTGTGG-------------------------------------------------------	20	-	-	2
	------------------------------GCCTGGGAAACTCTGTGTGGAAA----------------------------------------------------	23	-	-	1
	------------------------------------------------GGAAATAGGTGGTAAACCTG-------------------------------------	20	-	-	1
	-------------------------------------------TGTGTGGAAATAGGTGGTAA------------------------------------------	20	-	-	1
	---------------------------------TGGGAAACTCTGTGTGGAAATAG-------------------------------------------------	23	-	1	-
	------------------------------------GAAACTCTGTGTGGAAATAGGTGGT--------------------------------------------	25	-	-	1
	-----------------------------TGCCTGGGAAACTCTGTGTG--------------------------------------------------------	20	-	-	1
	------------------------------------------------GGAAATAGGTGGTAAACC---------------------------------------	18	-	-	1
rat	tcatggttgggctatgctagggctccagttgcctgggaaactctgtgTGGAAATAGGTGGTAAACCTGcctcactgtccctgttcatggggtgcctcatgatgat
	*********************************************************************************************************
	-------------------------------------------------------------------------------------------------------------------	ENSRNOT00000057552 ENSRNOG00000037879
rat	((((.((.((((.........((((((...((..((((......(((.((...(((((.....))))))).)))..)))).))...)))))))))).)).)))).	0.430 -34.80

rat

chromosome:4:119284132:119284236:-1

Opposite_strand|Intronic_coding|ENSRNOT00000057552|ENSRNOG00000037879 ## ENSRNOG00000037879|protein_coding||

novel_cloningHIGH_nonpairedHIGH_bulgeHIGH (11 loci)

block770131 [novel_cloningHIGH_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.167

no

no

0.40/0.48

20/24/1.00

0.0

0.0

30

0

5

0

12

0

3arm

1

nd

0.41

4

51

5

na

na

		reads	miRBase family seed
seed	----------------------------------------GCCUUGU--------------------------	39	novel
seed	--------------------------------------AUGCCUU----------------------------	9	miR-532
seed	----------------------------------------------UGGACUA--------------------	1	novel
seed	-----------------------------------------CCUUGUG-------------------------	1	novel
seed	---------------------------------------------GUGGACU---------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T4S1	T5S1
	---------------------------------------TGCCTTGTGGACTATTCTCAGT------------	22	1	2	2	2	30
	-------------------------------------TATGCCTTGTGGACTATTCTCAGT------------	24	-	-	-	-	7
	-------------------------------------TATGCCTTGTGGACTATTCTCA--------------	22	-	-	-	-	2
	--------------------------------------------TGTGGACTATTCTCAGTTAC---------	20	-	-	-	-	1
	---------------------------------------------GTGGACTATTCTCAGTTACCT-------	21	-	-	-	-	1
	---------------------------------------TGCCTTGTGGACTATTCTCA--------------	20	-	-	-	-	1
	---------------------------------------TGCCTTGTGGACTATTCTCAG-------------	21	-	-	-	-	1
	----------------------------------------GCCTTGTGGACTATTCTCAGT------------	21	-	-	-	-	1
rat	ggtctacagcatacatcggatctacggcacacccttttaTGCCTTGTGGACTATTCTCAGTtacctgtttcca
	*************************************************************************
rat	((...((((...((...((((((((((((..........))))..)))))....)))..))...))))..)).	0.680 -14.20

rat

chromosome:19:15931257:15931329:-1

intergenic

block896887 [novel_cloningHIGH_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.968

no

no

0.32/0.44

18/25/0.84

0.0

0.8

782

0

10

0

10

3

3arm

1

nd

0.50

4

1647

10

na

na

		reads	miRBase family seed
seed	-----------------------------------GCCCGAU------------------------	1377	novel
seed	------------------------------------CCCGAUG-----------------------	199	novel
seed	-------------------------------------CCGAUGA----------------------	70	novel
seed	----------------------------------UGCCCGA-------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	----------------------------------TGCCCGATGATTATAAAAAG------------	20	27	26	37	25	68	27	30	17	222	192
	----------------------------------TGCCCGATGATTATAAAAAGAC----------	22	22	10	23	19	42	13	37	29	172	128
	-----------------------------------GCCCGATGATTATAAAAAG------------	19	6	6	8	4	13	13	6	3	41	31
	----------------------------------TGCCCGATGATTATAAAAAGA-----------	21	2	5	3	5	6	3	2	6	27	29
	-----------------------------------GCCCGATGATTATAAAAAGAC----------	21	3	2	3	-	3	1	5	-	15	16
	----------------------------------TGCCCGATGATTATAAAA--------------	18	-	3	2	-	8	3	3	2	11	16
	------------------------------------CCCGATGATTATAAAAAG------------	18	-	1	1	1	4	-	5	1	15	10
	----------------------------------TGCCCGATGATTATAAAAAGACGC--------	24	-	-	-	2	-	1	3	1	18	10
	----------------------------------TGCCCGATGATTATAAAAA-------------	19	1	2	-	1	2	2	3	4	4	4
	------------------------------------CCCGATGATTATAAAAAGA-----------	19	1	1	1	-	1	-	-	1	5	7
	----------------------------------TGCCCGATGATTATAAAAAGACG---------	23	1	-	1	-	2	-	1	1	5	5
	------------------------------------CCCGATGATTATAAAAAGAC----------	20	-	1	-	-	-	-	-	-	7	7
	-----------------------------------GCCCGATGATTATAAAAAGA-----------	20	-	1	-	-	-	1	-	-	4	4
	-----------------------------------GCCCGATGATTATAAAAA-------------	18	-	-	-	-	1	-	-	-	2	1
	-----------------------------------GCCCGATGATTATAAAAAGACGC--------	23	-	-	-	-	-	-	1	-	-	2
	-----------------------------------GCCCGATGATTATAAAAAGACG---------	22	1	-	-	-	1	-	-	-	-	1
	----------------------------------TGCCCGATGATTATAAAAAGACGCG-------	25	-	-	-	-	1	-	-	-	-	-
	---------------------------------TTGCCCGATGATTATAAAAAGAC----------	23	-	-	-	1	-	-	-	-	-	-
rat	cttctatgagtagtttgtcagtgcagaagtctctTGCCCGATGATTATAAAAAGACgcgaaagagg
	******************************************************************
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000055144 ENSRNOG00000017158 Q5BJU2_RAT
	++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000023040 ENSRNOG00000017158 Q5BJU2_RAT
rat	(((((.((.((..(((((((..(((((....))).))....))))....)))..)).))..)))))	0.690 -8.10

rat

chromosome:1:168834461:168834526:-1

Same_strand|Intronic_coding|ENSRNOT00000023040|ENSRNOG00000017158 ## ENSRNOG00000017158|protein_coding|Q5BJU2_RAT|Eif4g2_predicted protein. [Source:UniProtKB/TrEMBL;Acc:Q5BJU2]

block1001781 [novel_cloningHIGH_nonpairedHIGH_bulgeHIGH]

Hairpin parameters

miRNA

randfold

RNA

rep

GC min/max

len min/max/mir_range

5'var

3'var

reads

reads edited

libs

libs edited

base distance

loop distance

position

locations

exon distance

unpaired

max bulge

total reads

total libs

dicer

drosha

no

0.326

no

no

0.47/0.53

18/21/0.83

0.0

0.0

74

0

10

0

24

3

3arm

1

nd

0.45

4

107

10

na

na

		reads	miRBase family seed
seed	------------------------------------------------------CAGGACU------------------------------------	101	novel
seed	-------------------------------------------------------AGGACUU-----------------------------------	4	novel
seed	---------------------------------------------------------GACUUGG---------------------------------	1	novel
seed	--------------------------------------------------------GGACUUG----------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
	-----------------------------------------------------ACAGGACTTGGAGTCAGAAG------------------------	20	1	3	6	1	7	2	12	19	14	16
	-----------------------------------------------------ACAGGACTTGGAGTCAGAAGT-----------------------	21	2	-	1	1	-	-	1	1	1	1
	-----------------------------------------------------ACAGGACTTGGAGTCAGAA-------------------------	19	-	-	-	-	2	3	-	2	1	-
	-----------------------------------------------------ACAGGACTTGGAGTCAGA--------------------------	18	-	-	-	1	-	-	-	1	1	1
	------------------------------------------------------CAGGACTTGGAGTCAGAAG------------------------	19	-	-	-	-	-	1	-	2	-	-
	------------------------------------------------------CAGGACTTGGAGTCAGAA-------------------------	18	-	-	-	-	-	-	-	-	-	1
	-------------------------------------------------------AGGACTTGGAGTCAGAAGT-----------------------	19	-	-	-	-	-	-	-	1	-	-
	--------------------------------------------------------GGACTTGGAGTCAGAAGT-----------------------	18	-	-	1	-	-	-	-	-	-	-
rat	gtacaccctaacacgctctagcagagggtggcataaaggctgcgttttggaacACAGGACTTGGAGTCAGAAGttgctgggtttgcatggtgttgct
	*************************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000030914 ENSRNOG00000025167 Sema4b
rat	(((((((....((.((.(((((((....((((...(((.(((.(((....))).)))..)))...))))....))))))))).))...))))).)).	0.810 -28.40

rat

chromosome:1:136019128:136019224:1

Same_strand|Intronic_coding|ENSRNOT00000030914|ENSRNOG00000025167 ## ENSRNOG00000025167|protein_coding|Sema4b|Sema4b protein (Fragment). [Source:UniProtKB/TrEMBL;Acc:Q4KM50]

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