Small RNA from different tissues of two rat strains.

miR-Intess™

miR classification

miR expression

block435912_cand

block435912_cand

Cloning frequencies
absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block435912_cand 3arm	0	0	10.500	7.500	0	0	0	0	0	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block435912_cand 3arm	0	0	0.001	0.000	0	0	0	0	0	0

block435912_cand relative cloning frequencies

block435912 [novel_lenNOK_cloningOK_randfoldOK]

Hairpin parameters
miRNA	randfold	RNA	rep	GC min/max	len min/max/mir_range	5'var	3'var	reads	reads edited	libs	libs edited	base distance	loop distance	position	locations	exon distance	unpaired	max bulge	total reads	total libs	dicer	drosha
no	0.001	no	no	0.48/0.55	18/23/0.17	0.0	0.7	21	0	2	0	26	4	3arm	2	nd	0.19	3	23	2	na	na

Families
Member of family miR-122 (seed GGAGUGU): rno-mir-122, rno-mir-541, block435912_cand, block1251548_cand, block1623670_cand, block2486258_cand

block435912 hairpin

		reads	miRBase family seed
seed	-----------------------------------------------------------GGAGUGU---------------------------------------	23	miR-122
		len	cloning frequencies
			T2S1	T2S2
	----------------------------------------------------------TGGAGTGTGACAGCTGTA-----------------------------	18	5	5
	----------------------------------------------------------TGGAGTGTGACAGCTGTAC----------------------------	19	6	3
	----------------------------------------------------------TGGAGTGTGACAGCTGTACGT--------------------------	21	2	-
	----------------------------------------------------------TGGAGTGTGACAGCTGTACG---------------------------	20	-	1
	----------------------------------------------------------TGGAGTGTGACAGCTGTACGTTT------------------------	23	-	1
rat	tgtgtattgtgagattaaccgtgttgacgtgagctgccattccatacagttaagctggTGGAGTGTGACAGCTGTACGTttctaattaaacttataaccccgcac
	*********************************************************************************************************
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000040505 ENSRNOG00000010721 Dlg7_predicted
	+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++	ENSRNOT00000015553 ENSRNOG00000010721 Dlg7_predicted
rat	((((..(((((((.((((...((..(((((((((((.((((((((.(((.....)))))))))))...))))).))))))..)).)))).)))))))...)))).	1.000 -34.50

rat

chromosome:15:23346938:23347042:-1

Same_strand|Intronic_coding|ENSRNOT00000015553|ENSRNOG00000010721 ## ENSRNOG00000010721|protein_coding|Dlg7_predicted|

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