Small RNA from different tissues of two rat strains.

miR-Intess™

miR classification

miR expression

block6229_cand

block6229_cand

Cloning frequencies
absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block6229_cand 3arm	2	2	0	1	1	0	1	1	2	0
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
block6229_cand 3arm	0.000	0.000	0	0.000	0.000	0	0.000	0.000	0.000	0

block6229_cand relative cloning frequencies

block6229 [novel_cloningOK_5varHIGH_mirtron_randfoldOK]

Hairpin parameters
miRNA	randfold	RNA	rep	GC min/max	len min/max/mir_range	5'var	3'var	reads	reads edited	libs	libs edited	base distance	loop distance	position	locations	exon distance	unpaired	max bulge	total reads	total libs	dicer	drosha
no	0.001	no	no	0.57/0.62	20/24/1.00	1.1	1.1	10	0	7	0	25	6	3arm	1	0	0.24	2	10	7	na	na

block6229 hairpin

		reads	miRBase family seed
seed	-----------------------------------------------------------------------AACCACC------------------------------------------	7	novel
seed	------------------------------------------------------------------------ACCACCC-----------------------------------------	2	novel
seed	----------------------------------------------------------------------------CCCUUGU-------------------------------------	1	novel
		len	cloning frequencies
			T1S1	T1S2	T2S2	T3S1	T4S1	T4S2	T5S1
	----------------------------------------------------------------------GAACCACCCTTGTCCCTTCCCTCA--------------------------	24	-	1	-	-	1	-	-
	-----------------------------------------------------------------------AACCACCCTTGTCCCTTCCCTCA--------------------------	23	-	-	-	-	-	-	2
	----------------------------------------------------------------------GAACCACCCTTGTCCCTTCCC-----------------------------	21	1	1	-	-	-	-	-
	----------------------------------------------------------------------GAACCACCCTTGTCCCTTCCCTC---------------------------	23	1	-	-	-	-	1	-
	----------------------------------------------------------------------GAACCACCCTTGTCCCTTCCCT----------------------------	22	-	-	1	-	-	-	-
	---------------------------------------------------------------------------ACCCTTGTCCCTTCCCTCAG-------------------------	20	-	-	-	1	-	-	-
rat	gtggttaactcctttcaaaatgcccagttgggaagggaggggtggggctgctaggtgggaatgactgctaGAACCACCCTTGTCCCTTCCCTCAGgggtgcacttggaaggcccgttcat
	************************************************************************************************************************
	>+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>	ENSRNOT00000008537 ENSRNOG00000006460 RGD1304601
rat	((((......((((((((..(((((....((((((((((((((((..((....(((((......)))))))..)))))))..)))))))))....)))))...)))))))).....))))	0.980 -54.40

rat

chromosome:10:13409755:13409874:-1

Same_strand|Intronic_coding|ENSRNOT00000008537|ENSRNOG00000006460 ## ENSRNOG00000006460|protein_coding|RGD1304601|Putative N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) (GlcNAc 6-P deacetylase) (Amidohydrolase domain-containing protein 2). [Source:UniProtKB/Swiss-Prot;Acc:Q5BJY6]

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