Small RNA from different tissues of two rat strains.

miR-Intess™

miR classification

miR expression

rno-let-7a-2-as

rno-let-7a-2-as

Cloning frequencies
absolute	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
rno-let-7a-2-as 3arm	2	2.500	0.500	1	0	0	0	0.500	2.500	2.500
normalized	T1S1	T1S2	T2S1	T2S2	T3S1	T3S2	T4S1	T4S2	T5S1	T5S2
rno-let-7a-2-as 3arm	0.000	0.000	0.000	0.000	0	0	0	0.000	0.000	0.000

rno-let-7a-2-as relative cloning frequencies

block2301627 (Antisense miRBase rno-let-7a-2-as) [miRBaseNonMapped_lenNOK_cloningOK]

Hairpin parameters
miRNA	randfold	RNA	rep	GC min/max	len min/max/mir_range	5'var	3'var	reads	reads edited	libs	libs edited	base distance	loop distance	position	locations	exon distance	unpaired	max bulge	total reads	total libs	dicer	drosha
miRBaseAS rno-let-7a-2-as	0.190	no	no	0.36/0.42	18/22/0.13	0.2	0.4	16	0	7	0	26	0	3arm	2	nd	0.28	1	23	7	na	na

block2301627 hairpin

		reads	miRBase family seed
seed	--------------------------------------------------------------------------------------UAUACAA------------------------------------------------	21	let-7b*
seed	------------------------------------------------------------------------------------ACUAUAC--------------------------------------------------	2	novel
		len	cloning frequencies
			T1S1	T1S2	T2S1	T2S2	T4S2	T5S1	T5S2
	-------------------------------------------------------------------------------------CTATACAACCTACTACCT--------------------------------------	18	3	3	-	1	1	4	2
	-------------------------------------------------------------------------------------CTATACAACCTACTACCTC-------------------------------------	19	-	2	1	1	-	1	1
	-----------------------------------------------------------------------------------AACTATACAACCTACTACCTCA------------------------------------	22	-	-	-	-	-	-	2
	-------------------------------------------------------------------------------------CTATACAACCTACTACCTCA------------------------------------	20	1	-	-	-	-	-	-
rat	-----------------GTTGCTTTGTGCAAGTCCCAAGGAAAGCTAGGAGGCTGTACAGTTATCTCCCTTGTTGTAACTCTAAACTATACAACCTACTACCTCAGCCTGGGAGCATGCCGTCACAGTGGC----------
human	-------TTCTCACCATGTTGTTTAGTGCAAGACCCAAGGAAAGCTAGGAGGCTGTACAGTTATCTCCCTTGATGTAATTCTAAACTATACAACCTACTACCTCAACCTGGGAGCATGCAGTCACAATGGCTGGAA-----
mouse	TAGCTCGTTCTTGCCATGTTGCTTTGTGCAAGTCCCAAGGAAAGCTAGGAGGCTGTACAGTTATCTCCCTTGATGTAACTCTAAACTATACAACCTACTACCTCAACCTGGGAACATGCAGTCACAATGGCTGGAGAGCTA
	** ***** *********************************** * ********************** *** * ** **
	..... ........<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<.......... .....	ENSRNOT00000053671 ENSRNOG00000035548 rno-let-7a-2
rat	((..((.((.(((.(((((.(((..((.(((.(((.((((.((((.....................)))).)))).))).))).))...)))))).)).)))...)).))..))	0.920 -29.80
human	.(((..((((..((....((((....(((.(((..((.(((.(((.((((.(((((((......)))).........))).)))).))).))).))...)))))).)))).....))..))))..))).	0.927 -29.80
mouse	((((((..(((.(((((((.((....((((..((((.(((..((.(((.(((.((((.(((((((......)))).........))).)))).))).))).))...)))))))...)))))).))).)))).)))))))))	0.985 -43.80

rat	chromosome:8:44525759:44525872:-1	Opposite_strand\|Exonic_non-coding\|ENSRNOT00000053671\|ENSRNOG00000035548 ## ENSRNOG00000035548\|miRNA\|rno-let-7a-2\|rno-let-7a-2 [Source:miRBase;Acc:MI0000828] ## {ASMIR: rno-let-7a-2-as}
human	chromosome:11:121522410:121522538:1	Opposite_strand\|Boundary_non-coding\|ENST00000362105\|ENSG00000198975\|miRNA\|hsa-let-7a-2 [Source:miRBase;Acc:MI0000061] ## {ASMIR: hsa-let-7a-2-as}
mouse	chromosome:9:41344779:41344919:-1	Opposite_strand\|Boundary_non-coding\|ENSMUST00000083680\|ENSMUSG00000065614\|miRNA\|mmu-let-7a-2 [Source:miRBase;Acc:MI0000557] ## {ASMIR: mmu-let-7a-2-as}

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