logoSmall RNA from different tissues of two rat strains.


Summary

InteRNA Genomics small RNA analysis pipeline miR-Intess™. Jan 23, 2009.


Total number of analysed libraries: 10

Total number of raw reads: 98,614,116

Total number of accepted reads: 60,757,867

Total number of mapped reads: 25,314,950

Total number of cloned miRBase miRNAs: 278

Total number of homolog miRBase miRNAs: 33

Total number of novel identified miRNAs: 50

Parameters of analysis
Reference organism: ratAssembly: RGSC3.4 
Ensembl database: rattus_norvegicus_core_52_34uUCSC database: rn4Mirbase version: 12.0
Min read length: 18Max read length: 30Min mapped read length: 18
READ trimming: THREE_PRIME_ALL_TRIMEditing analysis: 0 
Hairpin classification:
Distance from stem base: 5Distance from loop: 0Max distance between mature and star: 50
Min mature length: 20Max mature length: 24Relative read abundance for signature calculation:0.3
Max mature GC: 0.9Min Dicer/Drosha overhang: 1Max Dicer/Drosha overhang: 3
Min required reads for novel confident miRNA: 10Min required libs for novel confident miRNA: 2Lib/read overlap for confident novel miRNAs: AND
Randfold threshold for confident novel miRNAs: 0.01Species for conservation analysis: human mouseRNA forester threshold for homology: 0.3

©2008 InteRNA Genomics B.V.